High‐resolution imaging and nano‐manipulation of biological structures on surface
High‐resolution imaging and nano‐manipulation of biological structures on surface
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DOI：
10.1002/jemt.20925
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发表时间：
2011-07
影响因子：
2.5
通讯作者：
Yi Zhang;Xiaofang Hu;Jielin Sun;Yi Shen;Jun Hu;Xuemin Xu;Zhifeng Shao
中科院分区：
文献类型：
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作者：
Yi Zhang;Xiaofang Hu;Jielin Sun;Yi Shen;Jun Hu;Xuemin Xu;Zhifeng Shao
在这篇迷你评论中，我们讨论了我们最近的研究结果，通过原子力显微镜（AFM）成像和操纵生物大分子结构。在本综述的第一部分中，我们重点介绍了选定生物样本的高分辨率成像。膜蛋白的AFM图像揭示了与可识别的生物功能相关的详细构象特征。利用原子力显微镜成像系统地研究了在受控环境条件下短肽在不同基底上形成超分子结构的不同自组装行为。在第二部分中，我们提出了一种新的纳米操作技术，用于操作，分离，扩增和测序单个DNA分子，这可能会在分析困难的序列结构中找到独特的应用。最后，我们讨论了如何表征单个生物分子和活细胞的弹性。这些结果表明，原子力显微镜的高分辨能力不仅适合于解决某些生物学问题，而且还可以以其独特的优势应用于单分子分离和生物力学分析。Microsc. Res. Tech.，2011.© 2010 Wiley利斯公司
In this mini‐review we discuss our recent findings on imaging and manipulation of biological macromolecular structures by atomic force microscopy (AFM). In the first part of this review, we focus on high‐resolution imaging of selected biological samples. AFM images of membrane proteins have revealed detailed conformational features related to identifiable biological functions. Different self‐assembling behaviors of short peptides into supramolecular structures on various substrates under controlled environmental conditions have been systematically studied with AFM imaging. In the second part, we present a novel nano‐manipulation technique for manipulating, isolating, amplifying, and sequencing of individual DNA molecules, which may find unique applications in the analysis of difficult sequence structures. Finally, we discuss how to characterize the elasticity of individual biomolecules and live cells. These results demonstrate that not only the high resolution capacity of the AFM is suited to resolve certain biological questions, but can also be applied to single molecule isolation and biomechanical analysis with its unique advantages. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.