CYP52 (CYTOCHROME-P450ALK) MULTIGENE FAMILY IN CANDIDA-MALTOSA - IDENTIFICATION AND CHARACTERIZATION OF 8 MEMBERS
CYP52 (CYTOCHROME-P450ALK) MULTIGENE FAMILY IN CANDIDA-MALTOSA - IDENTIFICATION AND CHARACTERIZATION OF 8 MEMBERS
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DOI：
10.1089/dna.1995.14.163
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发表时间：
1995-02-01
影响因子：
3.1
通讯作者：
TAKAGI, M
中科院分区：
文献类型：
--
作者：
OHKUMA, M;MURAOKA, S;TAKAGI, M
在此之前，我们鉴定了三个基因，并提供了证据，证明了一个正构烷烃诱导细胞色素P450 （P450alk）多基因家族存在于一个正构烷烃同化二倍体型酵母，念珠菌麦芽糖。在本报告中，我们分离并鉴定了该基因家族的其他成员，包括总共13个p450alk相关序列（8个基因和5个等位基因）。两组，每组由两个基因组成，被串联在基因组中。一项基因替换实验表明，至少有一个基因在基因组中只有一个等位基因。测定的核苷酸和推断的氨基酸序列表明，它们都具有p450的特征成分，氨基酸同源性在94% ~ 37%之间。6个基因之间的相似性高于其他两个基因，因此被划分为一个亚家族。该亚家族的所有成员都被分配到同一条染色体上，表明序列相似性和染色体连锁之间具有良好的相关性。除1个基因外，其余基因均可由正构烷烃诱导，但其他脂肪碳源的诱导能力存在差异。
Previously, we characterized three genes and presented evidence for an n-alkane-inducible cytochrome P450 (P450alk) multigene family in an n-alkane-assimilating and diploid-type yeast, Candida maltosa. In the present report, we isolated and characterized additional members of this gene family, including a total of thirteen P450alk-related sequences (eight genes and five of their alleles). Two sets, each consisting of two genes, were tandemly arranged in the genome. A gene replacement experiment showed that at least one gene had only a single allele in the genome. The determined nucleotide and the deduced amino acid sequences indicated that all had a characteristic constituent for P450s and exhibited amino acid identities from 94% to 37% to each other. Six genes showed relatively higher similarities to each other than to the other two genes and were thus classified into a subfamily. All the members of this subfamily were assigned to the same single chromosome, showing a good correlation between sequence similarity and chromosomal linkage. Although all the genes except for one were induced by n-alkane, their inducibilities by some other aliphatic carbon sources showed variabilities.