Human endothelial progenitor cell-seeded hybrid graft:: Proliferative and antithrombogenic potentials in vitro and fabrication processing
Human endothelial progenitor cell-seeded hybrid graft:: Proliferative and antithrombogenic potentials in vitro and fabrication processing
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DOI：
10.1089/107632703762687609
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发表时间：
2003-02-01
影响因子：
--
通讯作者：
Matsuda, T
中科院分区：
文献类型：
--
作者：
Shirota, T;He, HB;Matsuda, T
在本文中，我们表明循环外周血中的人内皮祖细胞（EPC）可能是细胞掺入的工程血管移植物的新细胞来源。通过密度梯度技术收集的人外周血单核细胞的培养物形成了高度增殖的EPC集落，并随着培养时间的推移而扩大。内皮型一氧化氮合酶和6-酮前列腺素-F-1-α等抗血小板物质的生成率分别约为成熟内皮细胞(EC)的三分之一和约二分之一。另一方面，EPCs的组织型纤溶酶原激活剂产生率与ECs几乎相同。 EPCs被接种并培养在由涂有光反应性明胶层的微孔分段聚氨酯膜制成的小直径顺应性移植物（内径，1.5mm）上，随后通过离体循环承受流体动力剪切应力。完全覆盖移植物的 EPC 会伸长并与流动方向对齐，从而产生集成的 EPC 工程移植物。这些结果表明EPCs具有与ECs相当的高增殖潜力和高抗血栓形成潜力，是心血管组织工程的合适细胞来源。
In this article, we show that human endothelial progenitor cells (EPCs) in circulating peripheral blood may be a novel cell source for a cell-incorporated engineered vascular graft. Cultures of human peripheral blood mononuclear cells collected by the density gradient technique developed highly proliferative EPC colonies, which expanded with culture time. The production rates of antiplatelet substances such as endothelial-type nitric oxide synthase and 6-keto-prostaglandin-F-1-alpha were approximately one-third and approximately one-half of those of mature endothelial cells (ECs), respectively. On the other hand, the tissue-type plasminogen activator production rate of EPCs was almost the same as that of ECs. EPCs were seeded and cultured on a small-diameter compliant graft (inner diameter, 1.5 mm) made of microporous segmented polyurethane film coated with a photoreactive gelatin layer, and subsequently subjected to hydrodynamic shear stress by ex vivo circulation. EPCs fully covering the graft elongated and aligned themselves with the direction of the flow, resulting in the production of an integrated EPC-engineered graft. These results indicate that EPCs, which have high proliferative potential and high antithrombogenic potential, comparable to those of ECs, are a suitable cell source for cardiovascular tissue engineering.