Differential Pulse Voltammetric Determination of Two Antivirus Nucleoside Analogues in Blood of Rat
Differential Pulse Voltammetric Determination of Two Antivirus Nucleoside Analogues in Blood of Rat
复制标题
差示脉冲伏安法测定大鼠血液中两种抗病毒核苷类似物
DOI：
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发表时间：
2008
影响因子：
1.2
通讯作者：
Zhang Yue
中科院分区：
文献类型：
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作者：
Ni Yong-Nian;Zhang Yue
采用微分脉冲伏安法在乙二胺修饰玻碳电极上同时测定了大鼠代谢血中的两种抗病毒核苷类似物阿昔洛韦和阿昔洛韦，并对电极修饰和电极反应机理进行了探讨.在pH2.56的Britton-Robinson缓冲溶液中，阿昔洛韦和阿昔洛韦在修饰玻碳电极上均有一灵敏的不可逆氧化峰，峰电位分别为1.20 V和1.17 V，在最佳条件下，阿昔洛韦和阿昔洛韦的线性范围分别为0.20-4.0 mg/L和0.02-0.40 mg/L，检出限分别为77 μg/L和12 μg/L。采用主成分回归（PCR）和偏最小二乘法（PLS）对两种药物混合物的重叠光谱进行解析，方法简便、快速、灵敏、准确，可用于小鼠代谢血中两种抗病毒核苷类似物的测定。
Differential pulse voltammetry was applied to the simultaneous determination of two antivirus nucleoside analogues,aciclovir and penciclovir,in the rat metabolic blood at an ethylenediamine modified glassy carbon electrode.Moreover,the mechanism of the electrode modification and the electrode reaction was investigated and discussed.In the pH 2.56 Britton-Robinson buffer,both aciclovir and penciclovir had a sensitive irreversible oxidation peak on the modified glassy carbon electrode.The peak potentials were 1.20 V and 1.17 V for these two medicines,respectively.Under the optimal conditions,the peak current was linear with its concentration in the range of 0.20-4.0 mg/L for aciclovir and 0.02-0.40 mg/L for penciclovir.The detection limits were 77 μg/L for aciclovir and 12 μg/L for penciclovir.Furthermore,chemometrics of classical least squares(CLS),principal component regression(PCR) and partial least squares(PLS) were applied to resolve the overlap spectra of the admixture of the two medicines,without troublesome procedures of separation and purification.The proposed method has been applied to the determination of the two antivirus nucleoside analogues in the metabolic blood of mouse.