Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal Ions In Vivo Using High-Intensity Focused Ultrasound.
Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal Ions In Vivo Using High-Intensity Focused Ultrasound.
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DOI：
10.1021/jacs.1c11543
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发表时间：
2022-04-06
影响因子：
15
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Wang, Xiaojing;Kim, Gun;Chu, James L.;Song, Tingjie;Yang, Zhenglin;Guo, Weijie;Shao, Xiangli;Oelze, Michael L.;Li, King C.;Lu, Yi
在体内检测高时空分辨率的金属离子对于了解金属离子在健康和疾病状态下的作用至关重要。虽然利用光对金属离子传感器进行时空控制已经被证明，但由于在组织和体内缺乏穿透深度，限制了其应用。为了克服这一限制，我们在此报告了使用高强度聚焦超声(HIFU)远程传递按需的、时空分辨的热能，以在体外和体内激活目标区域的DNAzyme传感器。锌离子选择性DNAzyme探针被保护链灭活，以阻止催化酶结构的形成，然后通过HIFU诱导的局部温度升高来激活催化酶结构。通过这种设计，新的DNAzyme-HIFU探针已经在HeLa细胞和小鼠中证明了锌离子特异的荧光共振能量转移(FRET)成像。目前的方法可用于监测许多其他金属离子，用于活体成像和医疗诊断，使用已经获得或可以通过体外选择选择的金属特异性DNAzyme。
Detecting metal ions in vivo with a high spatiotemporal resolution is critical to understanding the roles of the metal ions in both healthy and disease states. Although spatiotemporal controls of metal-ion sensors using light have been demonstrated, the lack of penetration depth in tissue and in vivo has limited their application. To overcome this limitation, we herein report the use of high-intensity focused ultrasound (HIFU) to remotely deliver on-demand, spatiotemporally resolved thermal energy to activate the DNAzyme sensors at the targeted region both in vitro and in vivo. A Zn2+-selective DNAzyme probe is inactivated by a protector strand to block the formation of catalytic enzyme structure, which can then be activated by an HIFU-induced increase in the local temperature. With this design, Zn2+-specific fluorescent resonance energy transfer (FRET) imaging has been demonstrated by the new DNAzyme-HIFU probes in both HeLa cells and mice. The current method can be applied to monitor many other metal ions for in vivo imaging and medical diagnosis using metal-specific DNAzymes that have either been obtained or can be selected using in vitro selection.
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DOI：
10.1073/pnas.1310583110
发表时间：
2014-01-07
影响因子：
11.1
作者：
Chyan, Wen;Zhang, Daniel Y.;Radford, Robert J.
通讯作者：
Radford, Robert J.
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
10.8
作者：
Boissenot, Tanguy;Bordat, Alexandre;Tsapis, Nicolas
通讯作者：
Tsapis, Nicolas
影响因子：
9.3
作者：
Maret, Wolfgang
通讯作者：
Maret, Wolfgang
影响因子：
9.8
作者：
Brown, M. R. D.;Farquhar-Smith, P.;deSouza, N. M.
通讯作者：
deSouza, N. M.