TUBULAR CATABOLISM OF ALBUMIN IS ASSOCIATED WITH THE RELEASE OF AN INFLAMMATORY LIPID
TUBULAR CATABOLISM OF ALBUMIN IS ASSOCIATED WITH THE RELEASE OF AN INFLAMMATORY LIPID
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DOI：
10.1038/ki.1994.222
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发表时间：
1994-06-01
影响因子：
19.6
通讯作者：
SCHREINER, GF
中科院分区：
文献类型：
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作者：
KEESFOLTS, D;SADOW, JL;SCHREINER, GF
蛋白尿和肾小管间质炎症（TII）与人类肾小球肾炎进展为肾衰竭相关。各种形式的实验性肾病综合征与TII有关。为了研究TII的发生，我们利用白蛋白超载模型。大鼠腹腔注射牛血清白蛋白（BSA）1至14天，出现大量蛋白尿。在第3、7和14天，主要存在巨噬细胞间质浸润。大鼠尿液中含有巨噬细胞趋化因子，经乙酸乙酯萃取后分配到有机相中。TLC和HPLC特征为新型非极性脂质的特征。从培养物的近端小管（PT）节段后，在体内或体外暴露于高浓度的脂质充满BSA的上清液显示具有类似的色谱特性的趋化活性。用脱脂BSA培养PT几乎不产生活性。因此，这种炎症因子的产生是白蛋白携带的脂肪酸在肾小管代谢的结果，并可能导致蛋白尿相关TII的发生。
Proteinuria and tubulointerstitial inflammation (TII) correlate with progression to renal failure in human glomerulonephritis. Various forms of experimental nephrotic syndrome are associated with TII. To study the genesis of TII, we utilized the model of albumin overload. Rats received intraperitoneal bovine serum albumin (BSA) for 1 to 14 days, developing heavy proteinuria. A predominantly macrophage interstitial infiltrate was present at days 3, 7 and 14. The urine of the rats contained a factor chemotactic for macrophages which partitioned into the organic phase with ethyl acetate extraction. TLC and HPLC characteristics were those of a novel, non-polar lipid. Supernatant from the culture of proximal tubule (PT) segments after in vivo or in vitro exposure to high concentrations of lipid-replete BSA showed chemotactic activity with similar chromatographic characteristics. PT cultured with delipidated BSA produced little activity. Thus, the generation of this inflammatory factor occurs as a consequence of tubular metabolism of albumin-borne fatty acids and may contribute to the development of proteinuria-associated TII.