Nitric oxide production is increased in patients with rheumatoid arthritis but does not correlate with laboratory parameters of disease activity
Nitric oxide production is increased in patients with rheumatoid arthritis but does not correlate with laboratory parameters of disease activity
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DOI：
10.1016/s0009-8981(03)00324-3
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发表时间：
2003-10-01
影响因子：
5
通讯作者：
Choi, JW
中科院分区：
文献类型：
--
作者：
Choi, JW
背景资料：为了研究类风湿关节炎（RA）患者一氧化氮（NO）的产生是否与疾病活动的实验室参数相关，我们检测了115例RA患者的血清硝酸盐和亚硝酸盐（NOx）浓度、类风湿因子（RF）、C反应蛋白（CRP）、红细胞沉降率（ESR）和血清铁标志物。方法：采用Griess反应将硝酸盐还原为亚硝酸盐后测定血清NOx浓度。血清转铁蛋白受体（sTfR）和血清铁蛋白测定采用酶免疫法和化学发光法。评估CRP、ESR和RF与NOx浓度的相关系数。结果如下：无铁缺乏的RA患者（n = 72）血清NOx浓度平均为208.3 +/- 126.8 mumol/l，有铁缺乏的RA患者（n = 43）血清NOx浓度平均为267.9 +/- 148.4 mumol/l，显著高于健康对照组（分别为32.7 +/- 14.3 mumol/l，p < 0.01）。CRP < 0.7 mg/dl和CRP ≥ 3.0 mg/dl患者之间的平均NOx浓度无显著差异，ESR < 15.0 mm/h和ESR ≥ 40.0 mm/h患者之间的平均NOx浓度也无显著差异。血清NOx浓度与CRP、ESR和RF无显著相关性，但与血清铁标志物呈负相关。结论：RA患者的NO生成显著增加，然而，血清NOx浓度似乎与RA的疾病活动性无关，至少基于CRP、ESR和RE（C）2003 Elsevier B. V.的实验室数据。
Background: To investigate whether nitric oxide (NO) production correlates with laboratory parameters of disease activity in patients with rheumatoid arthritis (RA), we measured serum nitrates and nitrites (NOx) concentrations, rheumatoid factor (RF), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and serum iron markers in 115 RA patients. Methods: Serum NOx concentrations were determined after reduction of nitrates to nitrites using the Griess reaction. Serum transferrin receptor (sTfR) and serum ferritin were assayed using an enzyme immunoassay and a chemiluminescence method. Correlation coefficients of CRP, ESR, and RF vs. NOx concentrations were assessed. Results: Serum NOx concentrations averaged 208.3 +/- 126.8 mumol/l in RA patients without iron deficiency (n = 72) and 267.9 +/- 148.4 mumol/l in RA patients with iron deficiency (n - 43), which were significantly higher than those of healthy controls (32.7 +/- 14.3 mumol/l, p < 0.01, respectively). There were no significant differences in mean NOx concentrations between the patients with CRP < 0.7 mg/dl and with CRP greater than or equal to 3.0 mg/dl, nor between the patients with ESR < 15.0 mm/h and with ESR greater than or equal to 40.0 mm/h. Serum NOx concentrations showed no significant correlations with CRP, ESR, and RF but exhibited an inverse correlation with serum iron markers. Conclusion: NO production markedly increases in patients with RA, however, serum NOx concentration does not seem to be associated with disease activity of RA, at least on the basis of laboratory data of CRP, ESR, and RE (C) 2003 Elsevier B.V. All rights reserved.