Arabidopsis GIGANTEA protein is post-transcriptionally regulated by light and dark
Arabidopsis GIGANTEA protein is post-transcriptionally regulated by light and dark
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DOI：
10.1016/j.febslet.2006.01.016
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发表时间：
2006-02-01
期刊：
影响因子：
3.5
通讯作者：
Putterill, J
中科院分区：
文献类型：
--
作者：
David, KM;Armbruster, U;Putterill, J
巨型茶(Gi)是拟南芥光周期开花的关键调控因子，其编码的蛋白质没有已知的生化功能域。Gi基因的表达受生物钟的控制，但Gi蛋白的积累还没有被研究过。我们创造了产生功能性表位标记的GI的植物，使我们能够在日常周期中跟踪蛋白质。在这里，我们表明，当GI蛋白水平振荡时，结构性过表达或驱动其启动子，其积累是调节的日长以及阶段特定的因素。此外，我们还证明了GI蛋白振荡的机制之一是通过26S蛋白酶体在黑暗诱导的蛋白分解发生在翻译后。(C)2006年欧洲生化学会联合会。爱思唯尔出版，版权所有。
GIGANTEA (GI) is a key regulator of photoperiodic flowering in Arabidopsis and encodes a protein with no domains of known biochemical function. Expression of GI mRNA is controlled by the circadian clock, but GI protein accumulation has not been previously investigated. We generated plants that produced functional epitope-tagged GI to enable us to track the protein through the daily cycle. Here we show that GI protein levels oscillate when either constitutively overexpressed or driven by its promoter and that its accumulation is modulated by day length as well as by phase-specific factors. Also, we demonstrate that one of the mechanisms underlying GI protein oscillation occurs post-translationally via dark-induced proteolysis by the 26S proteasome. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.