INTERACTION OF BDELLOVIBRIO BACTERIOVORUS AND HOST BACTERIA .I. KINETIC STUDIES OF ATTACHMENT AND INVASION OF ESCHERICHIA COLI B BY BDELLOVIBRIO BACTERIOVORUS
INTERACTION OF BDELLOVIBRIO BACTERIOVORUS AND HOST BACTERIA .I. KINETIC STUDIES OF ATTACHMENT AND INVASION OF ESCHERICHIA COLI B BY BDELLOVIBRIO BACTERIOVORUS
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DOI：
10.1128/jb.95.3.744-753.1968
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发表时间：
1968-01-01
影响因子：
3.2
通讯作者：
SHILO, M
中科院分区：
文献类型：
--
作者：
VARON, M;SHILO, M
建立了噬菌蛭弧菌与宿主菌相互作用早期阶段的定量研究方法。附着测量基于宿主和寄生虫的差异过滤。通过估计机械搅拌后仍附着在宿主细胞上的放射性标记的蛭弧菌细胞来测量侵袭。附着动力学和最终附着的蛭弧菌细胞数取决于寄生虫的多样性、培养基的组成和pH以及孵育温度。蛭弧菌运动的抑制剂，包括螯合剂，NaN3，和低pH值，都抑制附着，厌氧。紫外线杀死的宿主细胞保留了它们附着蛭弧菌细胞的能力，而热杀死的细胞则失去了这种能力。这些抗生素对附着没有影响。
Quantitative methods were developed for the study of the early stages in the interaction ofBdellovibrio bacteriovorusand host bacteria. Attachment measurements were based on the differential filtration of host and parasite. Invasion was measured by estimation of radioactively labeledBdellovibriocells remaining attached to the host cells after mechanical agitation. The kinetics of attachment and the final number ofBdellovibriocells attached were dependent on the multiplicity of the parasite, the composition andpH of the medium, and the incubation temperature. Inhibitors ofBdellovibriomotility, including chelating agents, NaN3, and lowpH, all inhibited attachment, as did anaerobiosis. Ultraviolet-killed host cells retained their competence for attachment ofBdellovibriocells, whereas heat-killed cells lost it. Invasion was selectively inhibited by inhibitors of protein synthesis, such as streptomycin, puromycin, and chloramphenicol. These antibiotics had no effect on attachment.