Characterization of quantitative trait loci controlling genetic variation for preharvest sprouting in synthetic backcross-derived wheat lines
Characterization of quantitative trait loci controlling genetic variation for preharvest sprouting in synthetic backcross-derived wheat lines
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DOI：
10.1534/genetics.107.084939
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发表时间：
2008-03-01
期刊：
影响因子：
3.3
通讯作者：
van Ginkel, Maarten
中科院分区：
文献类型：
--
作者：
Imtiaz, Muhammad;Ogbonnaya, Francis C.;van Ginkel, Maarten
粗山羊草是小麦的野生近缘种，其种子休眠性比普通小麦强，是抗穗发芽性的重要组成部分。一个二倍体的Ae. lanschii登录号（AUS 18836）和四倍体（Triticum lurgidum L.）SSP.杜鲁姆湾Altar 84）小麦用于构建合成小麦（Syn37）。以277个抗/感Syn37/2 * Janz回交后代BC1F7为材料，研究了PHS的遗传结构。在三种环境中超过2年的SBL进行了评估，PHS通过三种措施进行了评估：发芽指数（GI），它测量谷物休眠，整个穗测定（SI），它考虑到所有穗形态，并计算视觉发芽种子蚂蚁：200（VI）。使用基于色度计和基于NaOH的方法测量谷物颜色。利用混合线性模型分析了PHSR和粒色的QTL及其加性效应、上位性效应和与环境的互作效应。复合区间作图后的单位点分析显示，在3DL和4AL染色体上，GI有4个QTL，SI有2个QTL，VI有4个QTL。第3DL染色体上的QPhs. dpiv-3D. 1位点与红粒色紧密连锁，距离为5cM。3D染色体上的另一个位点"QPhs.dpiv-3D.2"独立于RGC洛克斯。双位点分析检测到9个QTL的主效应和18个加性X加性互作GI，SI和VI。9个主效QTL中有2个和2个上位性QTL与环境互作显著。加性效应和上位性效应都对PHSR的表型变异有贡献，所鉴定的标记是在多个位点进行有利等位基因标记辅助选择的潜在候选者。SBL源自前Ae。lauschii被证明是一个很有前途的工具，解剖，渐渗，和金字塔不同的PHSR基因适应小麦遗传背景。PIIS抗性在SBL中的增强表达使我们能够从红粒Ae中培育出抗PIIS的白色小麦种质。劳氏加入
Aegilops lauschii, the wild relative of wheat, has stronger seed dormancy, a major component of preharvest sprouting resistance (PHSR), than bread wheat. A diploid Ae. lanschii accession (AUS18836) and a tetraploid (Triticum lurgidum L. ssp. durum, var. Altar84) wheat were used to construct a synthetic wheat (Syn37). The genetic architecture of PHS was investigated in 277 BC1F7 synthetic backcross lines (SBLs) derived from Syn37/2*Janz (resistant/susceptible). The SBLs were evaluated in three environments over 2 years and PHS was assessed by way of three measures: the germination index (GI), which measures grain dormancy, the whole spike assay (SI), which takes into account all spike morphology, and counted visually sprouted seeds ant: of 200 (VI). Grain color was measured using both Chroma Meter- and NaOH-based approaches. QTL for PHSR and grain color were mapped and their additive and epistatic effects as well as their interactions with environment were estimated by a mixed linear-model approach. Single-locus analysis following composite interval mapping revealed four QTL for GI, two QTL for SI, and four QTL for VI on chromosomes 3DL and 4AL. The locus QPhs.dpiv-3D.1 on chromosome 3DL was tightly linked to the red grain color (RGC) at a distance of 5 cM. The other locus on chromosome 3D, "QPhs.dpiv-3D.2" was independent: of RGC lochs. Two-locus analysis detected nine QTL with main effects and 18 additive X additive interactions for GI, SI, and VI. Two of the nine main effects QTL and two epistatic QTL showed significant interactions with environments. Both additive and epistatic effects contributed to phenotypic variance in PHSR and the identified markers are potential candidates for marker-assisted selection of favorable alleles at multiple loci. SBLs derived front Ae. lauschii proved to be a promising tool to dissect, introgress, and pyramid different PHSR genes into adapted wheat genetic backgrounds. The enhanced expression of PI IS resistance in SBLs enabled us to develop white PHS-resistant wheat germplasm Prom the red-grained Ae. lauschii accession.