Characterization of human papillomavirus type 13 from focal epithelial hyperplasia Heck lesions
Characterization of human papillomavirus type 13 from focal epithelial hyperplasia Heck lesions
复制标题
局灶性上皮增生 Heck 病变中 13 型人乳头瘤病毒的特征
DOI：
10.1128/jvi.47.2.363-366.1983
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发表时间：
1983
影响因子：
5.4
通讯作者：
Chilf
中科院分区：
文献类型：
--
作者：
H. Pfister;Inge Hettich;U. Runne;;and;N. Gabriel;Chilf
一名土耳其患者的局灶性上皮增生Heck病变显示含有乳头瘤病毒特异性DNA，该DNA被分子克隆到λ噬菌体中。经证实与人乳头瘤病毒（HPV）6型DNA和HPV 11型DNA有关。再结合动力学显示，交叉杂交的4和3%，分别。与HPV 1、2、3、4、5、8或10型无交叉反应性。这种乳头瘤病毒类型将被称为HPV 13型。DNA的特征在于用几种限制性内切酶切割，并对切割位点进行物理作图。从另外两个病例的丘疹病赫克含有HPV 13型DNA所示的Southern印迹杂交和特征性的切割模式。这可能表明HPV 13型与局灶性上皮增生Heck比其他HPV类型更频繁相关。
Focal epithelial hyperplasia Heck lesions of a Turkish patient were shown to contain papillomavirus-specific DNA, which was molecularly cloned into bacteriophage lambda. It proved to be related to human papillomavirus (HPV) type 6 DNA and HPV type 11 DNA. Reassociation kinetics revealed a cross-hybridization of 4 and 3%, respectively. There was no cross-reactivity with HPV type 1, 2, 3, 4, 5, 8, or 10. This papillomavirus type will be referred to as HPV type 13. The DNA was characterized by cleavage with several restriction enzymes, and the cleavage sites were physically mapped. Papules from two additional cases of Morbus Heck contained HPV type 13 DNA as shown by Southern blot hybridization and by the characteristic cleavage patterns. This may indicate that HPV type 13 is more frequently associated with focal epithelial hyperplasia Heck than are other HPV types.