Placental vascularization and apoptosis in Type-1 and gestational DM
Placental vascularization and apoptosis in Type-1 and gestational DM
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DOI：
10.1080/14767058.2016.1199676
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发表时间：
2017-05-01
影响因子：
1.8
通讯作者：
Buke, Baris
中科院分区：
文献类型：
--
作者：
Akarsu, Suleyman;Bagirzade, Mahmud;Buke, Baris
目的：本研究的目的是分析1型或妊娠期糖尿病mellitus.Methods患者的胎盘血管化和细胞凋亡的改变：胎盘样本取自正常（n=6），GDM（n=6），和1型糖尿病（n=6）妊娠冲洗PBS和固定在4%多聚甲醛。通过光学显微镜和电子显微镜检查获得的切片。随后，进行免疫组化染色，以评估细胞凋亡和血管形成与caspase-9和VEGF antibodies.Results：毛细血管结构在各种大小，在自由和干绒毛，观察到更密集的GDM组比在对照组和1型糖尿病组，利用电子显微镜。同样，与1型糖尿病和对照组相比，绒毛表面的微绒毛数量减少，更不规则和钝化。GDM组与1型糖尿病组和对照组相比，干绒毛、游离绒毛和内皮细胞的毛细血管免疫反应增强。VEGF免疫组化染色结果显示，1型糖尿病组和GDM组的VEGF免疫反应性较对照组强，尤其是在合体滋养细胞核和间质细胞核。结论：1型糖尿病和GDM胎盘绒毛间质毛细血管化程度增加，VEGF和caspase-9免疫反应增强，合胞体结节增多，可能继发于胎盘缺氧缺血。然而，需要更多的参与者来证实这些结论。
Objective: The objective of this study is to analyze the alteration in vascularization and apoptosis in the placentas of patients with Type 1 or gestational diabetes mellitus.Methods: Placental samples drawn from normal (n=6), GDM (n=6), and Type 1DM (n=6) pregnancies were rinsed in PBS and fixed in 4% paraformaldehyde. The obtained sections were examined by both light and electron microscopy. Subsequently, immunohistochemical staining was performed to evaluate apoptosis and vascularization with caspase-9 and VEGF antibodies.Results: Capillary structures in various sizes, both in free and in stem villi, were observed to be denser in the GDM group than in the control and Type-1DM groups, utilizing electron microscopy. Similarly, when compared with Type-1DM and controls, a decreased amount of microvilli with more irregularity and blunting on the villus surface was detected. GDM group showed increased immunoreactivity in capillaries of stem villi, free villi, and endothelial cells when compared with Type-1DM and control groups. Regarding the immunohistochemical staining with VEGF, Type-1DM, and GDM groups showed stronger immunoreactivity than the control group, especially in syncytiotrophoblastic cell nuclei and stromal cell nuclei. However, there was no significant difference between Type-1DM and GDM groups.Conclusion: Type-1DM and GDM placentas showed increased villous stromal capillarization, increased immunoreactivity with VEGF and caspase-9, and increased syncytial nodes, which may develop secondary to placental hypoxia-ischemia. However, more participants are needed to confirm these conclusions.