Upregulation of arginine vasopressin synthesis in the rat hypothalamus after kainic acid-induced seizures
Upregulation of arginine vasopressin synthesis in the rat hypothalamus after kainic acid-induced seizures
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DOI：
10.1016/j.brainres.2011.09.030
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发表时间：
2011-11-18
期刊：
影响因子：
2.9
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Iwanaga, Masaru;Ohno, Motoko;Ueta, Yoichi
我们使用原位杂交组织化学检查了红藻氨酸 (KA) 诱导的癫痫发作对正常大鼠室旁 (PVN) 和视上核 (SON) 精氨酸加压素 (AVP) 基因表达的影响。我们还研究了转基因大鼠 KA 诱导癫痫发作后 AVP 增强绿色荧光蛋白 (eGFP) 融合基因的表达。正常大鼠皮下注射 KA 后 3 小时和 24 小时，PVN 和 SON 中的 AVP 异核 (hn) RNA 水平显着增加。皮下注射后 3 小时、24 小时和 1 周时，PVN 和 SON 中的 AVP mRNA 水平没有显着变化。在正常大鼠中给予KA。在 KA 施用的转基因大鼠中，PVN 和 SON 的大细胞和小细胞分裂中的 AVP-eGFP 荧光明显强于载体施用的转基因大鼠。通过MK-801（非选择性N-甲基-D-天冬氨酸，NMDA，受体拮抗剂）预处理，AVP-eGFP转基因大鼠在给予KA后没有表现出惊厥前症状或惊厥，并且这些大鼠的PVN和SON的大细胞和小细胞分裂中的AVP-eGFP荧光显着减少。这些结果表明 MK801 可以阻止 KA 诱导的 AVP 转录本和 AVP 增加，因为癫痫发作活动被阻止或减少。 (C) 2011 Elsevier B.V. 保留所有权利。
We examined the effects of kainic acid (KA)-induced seizures on arginine vasopressin (AVP) gene expression in the paraventricular (PVN) and the supraoptic nuclei (SON) of normal rats using in situ hybridization histochemistry. We also investigated the expression of the AVP-enhanced green fluorescent protein (eGFP) fusion gene after KA-induced seizures in transgenic rats. AVP heteronuclear (hn) RNA levels in the PVN and the SON were significantly increased at 3 h and 24 h after subcutaneous (s.c.) administration of KA in normal rats. AVP mRNA levels in the PVN and the SON did not change significantly at 3 h, 24 h and 1 week after s.c. administration of KA in normal rats. In KA-administered transgenic rats, AVP-eGFP fluorescence in the magnocellular and parvocellular divisions of the PVN and the SON were significantly stronger compared to vehicle-administered transgenic rats. By pretreatment with MK-801 (nonselective N-methyl-D-aspartate, NMDA, receptor antagonist), AVP-eGFP transgenic rats after administration of KA did not show preconvulsive symptoms or convulsions and AVP-eGFP fluorescence in the magnocellular and parvocellular divisions of the PVN and the SON of these rats was significantly reduced. These results suggested that KA-induced increases in AVP transcripts and AVP were prevented by MK801 because seizure activity was prevented or reduced. (C) 2011 Elsevier B.V. All rights reserved.