AN ASSAY FOR SUPEROXIDE-DISMUTASE ACTIVITY IN MAMMALIAN TISSUE-HOMOGENATES
AN ASSAY FOR SUPEROXIDE-DISMUTASE ACTIVITY IN MAMMALIAN TISSUE-HOMOGENATES
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DOI：
10.1016/0003-2697(89)90192-9
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发表时间：
1989-05-15
影响因子：
2.9
通讯作者：
OBERLEY, LW
中科院分区：
文献类型：
--
作者：
SPITZ, DR;OBERLEY, LW
在测定大鼠乳腺组织超氧化物歧化酶（SOD）活性的过程中，注意到四氮唑蓝（NBT）和细胞色素c测定系统的干扰。这些干扰抑制了乳腺组织中SOD活性的准确测量，因此有必要开发一种新的基于NBT的测定方法，其中包括能够抑制组织特异性干扰的化合物。最有效的化合物是金属螯合剂，也是电子传递链抑制剂。其中以磺酸铜灵（BCS）效果最好。在NBT测定系统中加入BCS，可以准确测定有干扰的组织中的SOD活性。
During the course of measuring superoxide dismutase (SOD) activity in rat breast tissue, interferences in the nitrobule tetrazolium (NBT) and cytochrome c assay systems were noted. These interferences inhibit accurate measurement of SOD activity in breast tissues, necessitating the development of a new NBT-based assay that includes compounds capable of inhibiting tissue specific interferences. The most effective compounds were metal chelators that were also electron transport chain inhibitors. Bathocuproine sulfonate (BCS) was the most effective of these compounds. The inclusion of BCS in the NBT assay system was shown to make the accurate measurement of SOD activity in tissues with interferences possible.