Characterization of oligopeptide transporter (PepT1) in grass carp (Ctenopharyngodon idella)
Characterization of oligopeptide transporter (PepT1) in grass carp (Ctenopharyngodon idella)
复制标题
草鱼（Ctenopharyngodon idella）寡肽转运蛋白（PepT1）的表征
DOI：
10.1016/j.cbpb.2012.11.008
复制
发表时间：
2013-03-01
影响因子：
2.2
通讯作者：
Zhang, Jianshe
中科院分区：
文献类型：
--
作者：
Liu, Zhen;Zhou, Yi;Zhang, Jianshe
寡肽转运蛋白PepT1位于肠上皮刷状缘膜上，在蛋白质消化产生的二肽和三肽吸收中起重要作用。在这项研究中，我们从草鱼中克隆PepT1 cDNA，并表征其表达谱响应于膳食蛋白质和饲料添加剂（丁酸钠）处理。PepT1基因编码714个氨基酸的蛋白质，与其他脊椎动物同源物具有高度的序列相似性。表达分析显示草鱼前肠中PepT1 mRNA表达水平最高。此外，PepT1 mRNA的表达表现出昼夜变化，在所有三个肠段的肠在白天比夜间表达水平较低。在胚胎发育过程中，PepT1表现出动态的表达模式，在原肠胚阶段达到最高表达水平，在器官阶段达到最低水平。PepT1表达在孵化后14至34天显示恒定水平。为了确定不同蛋白质含量的鱼饲料是否对PepT1表达有任何影响，我们将研究扩展到PepT1表达的饮食调节。我们发现，膳食蛋白质水平对PepT1基因表达有显着影响。此外，PepT1的mRNA水平较高，饲喂鱼粉后比豆粕。此外，在体外和体内丁酸钠处理增加草鱼肠道PepT1的表达。研究结果表明，草鱼PepT1基因的表达在生长发育过程中存在时空调控，饲料蛋白质和饲料添加剂对草鱼PepT1基因的表达有显著影响。(c)2013 Elsevier Inc. All rights reserved.
The oligopeptide transporter (PepT1) is located on the brush-border membrane of the intestinal epithelium, and plays an important role in dipeptide and tripeptide absorptions from protein digestion. In this study, we cloned the PepT1 cDNA from grass carp and characterized its expression profile in response to dietary protein and feed additives (sodium butyrate) treatments. The PepT1 gene encodes a protein of 714 amino acids with high sequence similarity with other vertebrate homologues. Expression analysis revealed highest levels of PepT1 mRNA expression in the foregut of grass carp. In addition, PepT1 mRNA expression exhibited diurnal variation in all three bowel segments of intestine with lower levels of expression in daytime than nighttime. During embryonic development, PepT1 showed a dynamic pattern of expression reaching maximal levels of expression in the gastrula stage and minimal levels in the organ stage. The PepT1 expression showed constant levels from 14 to 34 day post-hatch. To determine whether fish diet of different protein contents may have any effect on PepT1 expression, we extended our research to dietary regulation of PepT1 expression. We found that dietary protein levels had a significant effect on PepT1 gene expression. In addition, PepT1 mRNA levels were higher after feeding with fish meal than with soybean meal. Moreover, in vitro and in vivo sodium butyrate treatments increased PepT1 expression in the intestine of grass carp. The results demonstrate for the first time that PepT1 mRNA expression is regulated in a temporal and spatial pattern during development, and dietary protein and feed additives had a significant effects on PepT1 gene expression in grass carp. (c) 2013 Elsevier Inc. All rights reserved.