MET oncogene aberrant expression in canine osteosarcoma
MET oncogene aberrant expression in canine osteosarcoma
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DOI：
10.1002/jor.1100180213
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发表时间：
2000-03-01
影响因子：
2.8
通讯作者：
Buracco, P
中科院分区：
文献类型：
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作者：
Ferracini, R;Angelini, P;Buracco, P
本研究的目的是探讨MET癌基因在犬骨肉瘤中的作用。七个大型犬自发性骨肉瘤的影响进行了整块肿瘤切除术。从冷冻的肿瘤样品中提取总RNA，并通过北方印迹分析评估MET癌基因的表达。七个活检样本中有五个表达高水平的MET癌基因;其在原发性肿瘤中的表达与先前在人类原发性骨肉瘤中鉴定的表达相当。其中一只狗的肺转移瘤表达的MET水平高于其原发肿瘤。自发性骨肉瘤在临床和病理学上与人类的相应疾病相似。我们先前证实MET癌基因在高比例的人骨肉瘤中异常表达。目前的研究结果还提供了狗和人类肿瘤之间的分子平行。这种体内模型可能有助于评估骨肉瘤治疗的新策略。
The objective of this study was to investigate the role of the MET oncogene in canine osteosarcoma. Seven large-breed dogs affected by spontaneous skeletal osteosarcoma underwent en bloc tumor excision. Total RNA was extracted from frozen tumor samples and assessed for expression of the MET oncogene by Northern blot analysis. Five of seven biopsy samples expressed high levels of the MET oncogene; its expression in the primary tumors was comparable with that previously identified in primary osteosarcomas in humans. A lung metastasis from one of the dogs expressed MET at a higher level than did its primary tumor. Spontaneously arising osteosarcoma in dogs clinically and pathologically mimics the corresponding disease in humans. We previously demonstrated that the MET oncogene was aberrantly expressed in a high percentage of human osteosarcomas. The results of the current study also provide a molecular parallel between the tumors in dogs and humans. This in vivo model may be helpful in evaluating new strategies for therapy against osteosarcoma.