Oxygen distribution in isolated perfused liver observed by phosphorescence imaging.
Oxygen distribution in isolated perfused liver observed by phosphorescence imaging.
复制标题
通过磷光成像观察离体灌注肝脏中的氧分布。
DOI:
10.1007/978-1-4684-5643-1_14
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发表时间:
1989
影响因子:
--
通讯作者:
Vanderkooi,JM
中科院分区:
文献类型:
--
作者:
Wilson,DF;Rumsey,WL;Vanderkooi,JM
The measurement of oxygen in intact tissues has proven to be full of technical difficulties. For example, the direct determination of oxygen in tissue using microelectrodes is subject to error in the low range of oxygen pressures (< 1 uM) and is restricted to fixed sites of evaluation (Silver, 1984). Moreover, insertion of the electrode damages the tissue and disturbs the local milieu. Indirect qualitative measurements using surface fluorescence of NADH indicate the presence of anoxia in tissue (Barlow and Chance, 1976) but lacks internal standardization (Koretsky et al., 1987). Spectrophotometric measurements of hemoglobin (Kekonen et al, 1987) and myoglobin (Fabel and Lubbers, 1965; Tamura et al., 1978) are useful for oxygen pressures near their P50but are subject to optical interference from changes in light scattering and from absorbtion by other chromophores. Alternatively, cryomicrospectrophotometry of myoglobin (Gayeski and Honig, 1986) can provide an accurate method for calculating intracellular oxygen pressure in some tissues. Because this method requires freezing of the tissue, it is impractical for monitoring changes in oxygenation during any length of time.
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影响因子:
--
作者:
J. Vanderkooi;David F. Wilson
通讯作者:
David F. Wilson
影响因子:
3.9
作者:
Mamoru Tamura;Nozomu Oshino;Britton Chance;Ian A. Silver
通讯作者:
Ian A. Silver
DOI:
--
发表时间:
--
期刊:
影响因子:
--
作者:
Fukuba Y;A Kondo;Watanabe S;Kikugawa Y;Endo M;Miura A;Hayashi N;Fukuoka Y;Koga S.;古賀 俊策;古賀 俊策
通讯作者:
古賀 俊策
DOI:
--
发表时间:
1988
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Wilson,DF;Rumsey,WL;Green,TJ;Vanderkooi,JM
通讯作者:
Vanderkooi,JM
影响因子:
--
作者:
D. Wilson;J. Vanderkooi;T. J. Green;G. Maniara;S. Defeo;D. Bloomgarden
通讯作者:
D. Bloomgarden