Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys
Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys
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DOI：
10.1016/j.vaccine.2005.09.032
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发表时间：
2006-02-27
期刊：
影响因子：
5.5
通讯作者：
Porter, KR
中科院分区：
文献类型：
--
作者：
Blair, PJ;Kochel, TJ;Porter, KR
本文报道了一种表达登革病毒3型前膜和包膜基因的DNA疫苗在猕猴体内的免疫原性和保护效果。通过ELISA和体外病毒中和测定，6只接种疫苗的动物中有5只表现出中度DEN特异性抗体应答。相比之下，六只对照动物中没有一只产生可检测到的抗DEN抗体。当5只接种疫苗的动物用活DEN-3病毒攻击并通过PCR扩增血清样品中的病毒RNA确定病毒血症时，一只动物完全受到保护，两只动物部分受到保护，如病毒血症平均天数减少所示。结果证明DEN-3 DNA疫苗能够引发中和抗体应答并部分保护免受活病毒攻击。这些发现支持在四价DNA疫苗中包含该构建体。爱思唯尔有限公司出版
A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine. Published by Elsevier Ltd.