Effect of desensitising paste containing 8% arginine and calcium carbonate on biofilm formation of Streptococcus mutans in vitro
Effect of desensitising paste containing 8% arginine and calcium carbonate on biofilm formation of Streptococcus mutans in vitro
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效果%20of%20脱敏%20膏%20含%208%%20精氨酸%20和%20钙%20碳酸盐%20on%20生物膜%20形成%20of%20链球菌%20mutans%20in%20体外
DOI：
10.1016/j.jdent.2013.04.013
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发表时间：
2013-07-01
影响因子：
4.4
通讯作者：
Sun, Hualing
中科院分区：
文献类型：
--
作者：
Fu, Dongjie;Pei, Dandan;Sun, Hualing
Objectives: To evaluate the influence of desensitising paste containing 8% arginine and calcium carbonate (Ar-Ca) on biofilm formation on dentine.Methods: Dentine discs were cut from extracted third molars and divided into the following three groups: no treatment, pumice treatment and Ar-Ca treatment. Surface topography and roughness were examined using scanning electron microscopy (SEM) and non-contact 3D surface profiler. After sterilisation, samples were incubated with Streptococcus mutans (S. mutans) for 4 h, 24 h and 72 h. Bacterial adhesion and biofilm formation were analysed using SEM, whereas MTT and lactic acid production assays were used to analyse the metabolic activity of S. mutans.Results: After polishing with either pumice or Ar-Ca, the surfaces of the samples became smoother than in the control group. The Ra values of the three experimental groups decreased significantly to 0.43 mu m, 0.3 mu m and 0.26 mu m, respectively. Compared to the control group, fewer bacteria adhered to the dentine surface in the Ar-Ca group, while biofilm thickness decreased significantly for both groups after incubating for 24 h and 72 h. MTT and lactic acid production levers also showed a significant reduction in the Ar-Ca group.Conclusions: Ar-Ca appears to present antibiofilm efficacy and may provide a promising approach to combat bacterial infection in hypersensitive dentinal lesions. Clinical significance: As a clinical application of desensitising polishing paste, the paste containing 8% arginine and calcium carbonate could also inhibit the biofilm formation effectively. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
