One-year in vitro stability of cardiac troponins and galectin-3 in different sample types
One-year in vitro stability of cardiac troponins and galectin-3 in different sample types
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DOI：
10.1016/j.cca.2017.11.018
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发表时间：
2018-01-01
影响因子：
5
通讯作者：
Mueller, Thomas
中科院分区：
文献类型：
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作者：
Egger, Margot;Dieplinger, Benjamin;Mueller, Thomas
背景资料：我们评估了长期在体外稳定性的心肌肌钙蛋白和galectin-3在不同的storage conditions.Methods：我们使用高灵敏度的心肌肌钙蛋白I（hs-cTnI）和galectin-3检测雅培和高灵敏度的心肌肌钙蛋白T（hs-cTnT）测定罗氏。在以下储存条件下，测量30名患者肝素处理的血浆、EDTA处理的血浆和血清样本中的分析物浓度：1）血液采集后立即用于基线测量的样本; 2）一次冻融循环后在-80 ℃下储存0.5年的样本; 3）两次冻融循环后在-80 ℃下储存1.0年的样本;和4）在一次冻融循环后在-80 ℃下储存1.0年的样品。结果：基线hs-cTnI、hs-cTnT和galectin-3的浓度范围分别为2.3 ~ 5436 ng/L、5.3 ~ 850 ng/L和8.3 ~ 79.3 ng/mL。在应用分析物稳定性的默认标准后，即使每种样品类型经过两次冻融循环，三种分析物也可稳定至少1.0年。我们观察到储存后分析物浓度相对于基线值的以下变化：心肌肌钙蛋白结果的四分位数间距（IQR）从约80%至115%，半乳糖凝集素-3结果的IQR从约结论：hs-cTnI、hs-cTnT和galectin-3在报告的储存条件下稳定，与所用样品类型无关。然而，我们观察到样本储存后分析物浓度的相当大的变化，这可能会影响这些分析物在使用冷冻血液样本的个体患者中的诊断/预后价值。
Background: We assessed the long-term in vitro stability of cardiac troponins and galectin-3 under different storage conditions.Methods: We used high-sensitivity cardiac troponin I (hs-cTnI) and galectin-3 assays from Abbott and a high-sensitivity cardiac troponin T (hs-cTnT) assay from Roche. The analyte concentrations of 30 patients were measured in heparin-treated plasma, EDTA-treated plasma and serum samples after the following storage conditions: 1) samples used immediately after blood collection for baseline measurements; 2) samples stored for 0.5 year at -80 degrees C after one freeze-thaw cycle; 3) samples stored for 1.0 year at -80 degrees C after two freeze-thaw cycles; and 4) samples stored for 1.0 year at -80 degrees C after one freeze-thaw cycle. According to the concept of acceptable change limits (ACL) the analytes were considered stable as long as the median concentration at a particular time point was > 80%.Results: Baseline hs-cTnI, hs-cTnT and galectin-3 concentrations ranged from 2.3 to 5436 ng/L, from 5.3 to 850 ng/L, and from 8.3 to 79.3 ng/mL, respectively. After applying the default criterion for analyte stability, the three analytes were stable for at least 1.0 year even after two freeze-thaw cycles for each sample type. We observed the following variation in analyte concentrations after storage relative to the baseline values: the interquartile range (IQR) of cardiac troponin results extended from approx. 80 to 115%, and the IQR of galectin-3 results extended from approx. 90 to 110%.Conclusion: hs-cTnI, hs-cTnT and galectin-3 were stable under the reported storage conditions irrespective of the sample type used. However, we observed a considerable variation in analyte concentrations after sample storage, which might affect the diagnostic/prognostic value of these analytes in individual patients using frozen blood samples.