Variation in the onset of CO2-induced anxiety in female Sprague Dawley rats
Variation in the onset of CO2-induced anxiety in female Sprague Dawley rats
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DOI：
10.1038/s41598-019-55493-0
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发表时间：
2019-12-12
影响因子：
4.6
通讯作者：
Weary, Daniel M.
中科院分区：
文献类型：
--
作者：
Amendola, Lucia;Ratuski, Anna;Weary, Daniel M.
二氧化碳（CO2）通常用于杀死实验室大鼠。大鼠发现CO2厌恶和厌恶因人而异，表明大鼠对CO2的敏感性不同。健康人在类似于大鼠避免的浓度下经历焦虑感，并且这些感觉通过苯二氮卓类药物的施用而减弱。我们的目的是评估苯二氮卓咪达唑仑对大鼠厌恶CO2的个体阈值的影响。6只雌性Sprague道利大鼠反复暴露于CO2逐渐填充的接近-回避测试。前三次暴露为对照治疗，随后三次暴露为咪达唑仑（0.375 mg/kg）。在每次治疗中，对CO2的厌恶不受暴露次数的影响;然而，耐受性从对照期间平均避免10.7% CO2增加到咪达唑仑治疗时避免15.5% CO2。这些结果表明，大鼠在暴露于CO2时会感到焦虑，并且大鼠CO2敏感性的变化是由这些焦虑感发作的个体差异驱动的。没有大鼠耐受诱导意识丧失所需的CO2浓度。
Carbon dioxide (CO2) is commonly used to kill laboratory rats. Rats find CO2 aversive and aversion varies between individuals, indicating that rats vary in CO2 sensitivity. Healthy humans experience feelings of anxiety at concentrations similar to those avoided by rats, and these feelings are diminished by the administration of benzodiazepines. Our aim was to assess the effects of the benzodiazepine midazolam on individual thresholds of rat aversion to CO2. Six female Sprague Dawley rats were repeatedly exposed to CO2 gradual-fill in approach-avoidance testing. The first three exposures were to a control-treatment followed by three exposures to midazolam (0.375 mg/kg). Within each treatment aversion to CO2 was not affected by exposure number; however, tolerance increased from an average of 10.7% CO2 avoided during control sessions, to 15.5% CO2 avoided when treated with midazolam. These results indicate that rats experience anxiety when exposed to CO2, and that variation in rat CO2 sensitivity is driven by individual differences in the onset of these feelings of anxiety. No rat tolerated CO2 concentrations required to induce loss of consciousness.