Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells
Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells
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Plexin-B1是宫颈癌中miR-214的靶标，促进HeLa细胞的生长和侵袭
DOI：
10.1016/j.biocel.2011.01.002
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发表时间：
2011-04-01
影响因子：
4
通讯作者：
Tang, Hua
中科院分区：
文献类型：
--
作者：
Qiang, Ran;Wang, Fang;Tang, Hua
据报道，丛蛋白-B1是Sema 4D的受体，可在各种类型的肿瘤细胞中引发多种，有时是相反的细胞反应。它参与调节肿瘤细胞的存活、增殖、血管生成、侵袭和转移。然而，plexin-B1基因在宫颈癌中的表达及其调控机制尚不清楚。目前的研究表明，丛蛋白-B1是过度表达的宫颈肿瘤组织相比，正常宫颈组织的免疫组化。Western blotting和定量RT-PCR。plexin-B1的表达与宫颈癌的侵袭转移密切相关。丛蛋白B1还促进人宫颈癌HeLa细胞的增殖、迁移和侵袭。我们还发现，丛蛋白B1水平与宫颈癌组织和HeLa细胞中的miR-214量呈负相关。miR-214的表达水平与宫颈癌的侵袭转移有关。此外，我们证明了在HeLa细胞中，丛蛋白B1通过丛蛋白B1的3 'UTR内的miR-214结合位点被miR-214抑制。miR-214的异位表达可抑制HeLa细胞的增殖能力、迁移能力和侵袭能力。我们的研究结果表明，plexin-B1，miR-214的目标，可能作为一个致癌基因在人类宫颈癌HeLa细胞。(C)2011爱思唯尔有限公司版权所有。
Plexin-B1, the receptor for Sema4D, has been reported to trigger multiple and sometimes opposing cellular responses in various types of tumor cells. It has been implicated in the regulation of tumor-cell survival, proliferation, angiogenesis, invasion and metastasis. However, the plexin-B1 gene expression and its regulatory mechanism in cervical cancer remain unclear. The present study shows that plexin-B1 is over-expressed in cervical tumor tissues compared to normal cervical tissues by immunohistochemistry. Western blotting and quantitative RT-PCR. The expression of plexin-B1 is significantly associated with cervical tumor metastasis and invasion according to the analysis of the clinicopathologic data. Plexin-B1 also promotes proliferation, migration and invasion in human cervical cancer HeLa cells. We also found that the plexin-B1 levels are inversely correlated with miR-214 amounts in both cervical cancer tissues and HeLa cells. And miR-214 expression level is also associated with metastasis and invasion of cervical tumor. Furthermore, we demonstrate that plexin-B1 is inhibited by miR-214 through a miR-214 binding site within the 3'UTR of plexin-B1 in HeLa cells. Ectopic expression of miR-214 could inhibit the proliferation capacity, migration and invasion ability of HeLa cells. Our findings suggest that plexin-B1, a target of miR-214, may function as an oncogene in human cervical cancer HeLa cells. (C) 2011 Elsevier Ltd. All rights reserved.