Latency competence of thirteen HSV-1 temperature-sensitive mutants.
Latency competence of thirteen HSV-1 temperature-sensitive mutants.
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13 种 HSV-1 温度敏感突变体的潜伏期能力。
DOI：
10.1099/0022-1317-49-1-149
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Subak-Sharpe,JH
中科院分区：
文献类型：
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作者：
Watson,K;Stevens,JG;Cook,ML;Subak-Sharpe,JH
分析了HSV-1的13种温度敏感（ts）突变体在小鼠脑中建立潜伏感染的能力。11个突变体可以被归类为潜伏期阳性或阴性;两个不能被分配到任何一组。突变体在大脑中的泄漏和粒子/感染性比率的差异被发现在结果中不起作用。选定的潜伏阴性突变体的Ts+回复突变体恢复了建立潜伏感染的能力，表明这是在这些代理人参与潜伏期。用各种突变体感染并保持在限制性温度下的神经母细胞瘤细胞的超微结构研究表明，建立潜伏感染的能力和各种形态学可识别的病毒产物的合成之间没有绝对的相关性。最后，从潜伏特性与先前建立的突变体的多肽表型的比较中得出结论，一个立即早期和一个或多个后期病毒功能对于建立和/或维持潜伏状态是必要的。
Thirteen temperature-sensitive (ts) mutants of HSV-1 were analysed for their capacity to establish latent infections in the brains of mice. Eleven of the mutants could be classified as latency-positive or -negative; two could not be assigned to either group. Leakiness of mutants in the brain and differences in particle/infectivity ratios were found not to play a role in the results.Ts+revertants of selected latency-negative mutants regained the capacity to establish latent infections, indicating that it was thetslesion in these agents which was involved in latency. Ultrastructural studies of neuroblastoma cells infected with various mutants and maintained at the restrictive temperature showed that no absolute correlations could be made between capacity to establish latent infection and synthesis of various morphologically identifiable virus products. Finally, from a comparison of latency characteristics with previously established polypeptide phenotypes of the mutants it was concluded that one immediate early and one or more later virus functions are necessary for establishment and/or maintenance of the latent state.