Identification of serum CCL15 in hepatocellular carcinoma.
Identification of serum CCL15 in hepatocellular carcinoma.
复制标题
肝细胞癌血清CCL15的鉴定
DOI：
10.1038/bjc.2012.494
复制
发表时间：
2013-01-15
影响因子：
8.8
通讯作者：
Guo, H.
中科院分区：
文献类型：
--
作者：
Li, Y.;Wu, J.;Zhang, W.;Zhang, N.;Guo, H.
背景：肝细胞癌是最致命的癌症之一，早期血清检测对提高治疗水平至关重要。肝炎感染是HCC的主要原因。方法：在本研究中，我们收集了80例HCC合并HBV(+)/肝硬化（+）患者、80例良性疾病患者（50例肝硬化患者和30例HBV感染患者）和60例健康对照组的知情同意的总血清样本。分析采用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）寻找新的HCC血清标志物。采用SDS-PAGE分离SELDI峰，LC-MS/MS鉴定，免疫组化（IHC）验证。采用迁移和侵袭实验检测细胞在体外的迁移和侵袭能力。结果：SELDI-TOF-MS在HCC患者的血清样本中发现了7777 M/Z的条带，而在健康对照和良性疾病患者的血清样本中没有发现。蛋白质组学特征中的蛋白（7777.27 M/Z）经肽质量指纹图谱鉴定为CC motif趋化因子15 （CCL15）。在HCC患者中检测到血清CCL15显著升高。功能分析表明，HCC细胞表达CCL15，从而促进HCC细胞的迁移和侵袭。结论：CCL15可能是HCC特异性蛋白质组生物学标志物，在肿瘤发生和肿瘤侵袭过程中具有重要作用。
Background:Early serum detection is of critical importance to improve the therapy for hepatocellular carcinoma (HCC), one of the most deadly cancers. Hepatitis infection is a leading cause of HCC.Methods:In the present study, we collected total serum samples with informed consent from 80 HCC patients with HBV (+)/cirrhosis (+), 80 patients with benign diseases (50 liver cirrhosis patients and 30 HBV-infected patients) and 60 healthy controls. Analysis was by using surface-enhanced laser desorption/ionisation-time-of-flight mass spectroscopy (SELDI-TOF-MS) to find new serum markers of HCC. SELDI peaks were isolated by SDS–PAGE, identified by LC-MS/MS and validated by immunohistochemistry (IHC) in liver tissues. Migration and invasion assay were performed to test the ability of cell migration and invasion in vitro.Results:SELDI-TOF-MS revealed a band at 7777 M/Z in the serum samples from HCC patients but not from healthy controls or patients with benign diseases. The protein (7777.27 M/Z) in the proteomic signature was identified as CC motif chemokine 15 (CCL15) by peptide mass fingerprinting. A significant increase in serum CCL15 was detected in HCC patients. Functional analysis showed that HCC cell expressed CCL15, which in turn promoted HCC cell migration and invasion.Conclusion:CCL15 may be a specific proteomic biomarker of HCC, which has an important role in tumorigenesis and tumour invasion.
登录
查看更多内容
影响因子：
7
作者：
Sheng, S;Chen, D;Van Eyk, JE
通讯作者：
Van Eyk, JE
影响因子：
4.4
作者：
Richter, R;Bistrian, R;Forssmann, U
通讯作者：
Forssmann, U
影响因子：
3.4
作者：
Blanc, JF;Lalanne, C;Rosenbaum, J
通讯作者：
Rosenbaum, J
影响因子：
3.5
作者：
Fujiyama, S;Tanaka, M;Tomita, K
通讯作者：
Tomita, K
影响因子：
2.4
作者：
Kwon SH;Ju SA;Kang JH;Kim CS;Yoo HM;Yu R
通讯作者：
Yu R