Measurement of Tumor Necrosis Factor and Lymphotoxins.
Measurement of Tumor Necrosis Factor and Lymphotoxins.
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肿瘤坏死因子和淋巴毒素的测量。
DOI：
10.1002/cpim.23
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发表时间：
2017
影响因子：
--
通讯作者：
Vogel,StefanieN
中科院分区：
文献类型：
--
作者：
Hogan,MMichele;Vogel,StefanieN
肿瘤坏死因子（TNF）超家族细胞因子在免疫应答的各个方面起着关键作用。肿瘤坏死因子（TNF）和光毒素（LT），LTα和LTβ，作为淋巴器官发育的主要效应细胞因子和介质特别重要。测量TNF和LTα活性的经典方法之一是通过证明它们裂解某些靶细胞的能力。一个详细的协议，用于测量这种活动使用一个高度敏感的指示细胞系。最近，已经开发了ELISA测定法来测量任何类型的生物流体中这些细胞因子的蛋白质浓度。John Wiley & Sons，Inc.
The tumor necrosis factor (TNF) superfamily of cytokines plays critical roles in all aspects of the immune response. TNF and the lymphotoxins (LT), LTα and LTβ, are particularly important as major effector cytokines and mediators or lymphoid organ development. One of the classical methods for the measurement of TNF and LTα activity is by demonstrating their ability to lyse certain target cells. A detailed protocol for the measurement of this activity using a highly sensitive indicator cell line is presented. More recently, ELISA assays have been developed to measure the protein concentration of these cytokines in any type of biologic fluid. © 2017 by John Wiley & Sons, Inc.