The SNPs in the promoter regions of the bovine FADS2 and FABP4 genes are associated with beef quality traits
The SNPs in the promoter regions of the bovine FADS2 and FABP4 genes are associated with beef quality traits
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DOI：
10.1016/j.livsci.2014.02.016
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发表时间：
2014-05-01
影响因子：
1.8
通讯作者：
Sasazaki, Shinji
中科院分区：
文献类型：
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作者：
Matsumoto, Hirokazu;Nogi, Takuya;Sasazaki, Shinji
众所周知，遗传因素会影响肉牛的肉质。脂肪代谢途径中脂肪酸去饱和酶2(FADS2)和脂肪酸结合蛋白4(FABP4)基因的表达已被报道影响脂肪相关性状。本研究旨在发现牛群体中FADS2和FABP4基因启动子区域的DNA多态性，以开发遗传标记。结果在FADS2启动子(FADS2 g.-823G>；A)和FABP4启动子(FABP4 g.-295A>；G和FABP4 g.-287A>；G)上发现了两个连锁SNP。所有这些SNP都被认为影响转录因子的结合位点，表明这些SNPs影响基因的表达和牛肉的品质。因此，我们进行了基因分型与性状的关联分析。结果表明，FADS2 g.-823G>；A对牛肉大理石纹评分(BMS)等性状有显著影响。荷斯坦群体中G/G动物的BMS(2.36)高于G/A动物(2.13)。在日本黑人群体中也观察到了同样的趋势。另一方面，虽然FABP4 SNPs对一些性状也有显著影响，但对本研究分析的任何性状都没有表现出加性效应。我们的结果表明，FADS2 G.-823G>；A是一个有用的遗传标记，用于肉牛育种。(C)2014爱思唯尔B.V.保留所有权利。
Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.