An effective strategy for preparing macroporous and self-healing bioactive hydrogels for cell delivery and wound healing
An effective strategy for preparing macroporous and self-healing bioactive hydrogels for cell delivery and wound healing
复制标题
制备用于细胞递送和伤口愈合的大孔和自愈生物活性水凝胶的有效策略
DOI：
10.1016/j.cej.2021.130677
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发表时间：
2021
影响因子：
15.1
通讯作者：
Haiyan Li
中科院分区：
文献类型：
--
作者：
Xin Zhang;Ying Li;Dan He;Zhijie Ma;Kai Liu;Ke Xue;Haiyan Li
大孔水凝胶在细胞输送和组织再生方面的应用越来越受到人们的关注。组装微凝胶是制备大孔水凝胶的有效方法。然而，目前的方法限于对装置和水凝胶固有性质的特定要求。此外，由于微凝胶的永久共价交联，大多数大孔水凝胶缺乏自修复能力。在这项研究中，我们提出了一种有效的策略，制备生物活性的大孔自愈合水凝胶通过挤压通过钢网和组装的MeHA-SABA的甲基丙烯酸化透明质酸（MeHA）和3-氨基苯基硼酸改性海藻酸钠（SABA）纳米多孔水凝胶（BG）离子产品创建的碱性条件下，通过钢丝网和MeHA-SABA微凝胶。结果表明，所获得的大孔水凝胶具有比纳米孔水凝胶大得多的孔径，这有利于细胞的浸润、存活和增殖。同时，大孔水凝胶具有良好的自修复能力，形成了动态的B-O键。此外，由于BG的生物活性，水凝胶可以诱导细胞迁移和细胞和血管的向内生长。因此，水凝胶的生物活性和大孔结构可以协同作用以刺激组织再生。该方法简单有效，可广泛应用于制备双组分自愈合大孔水凝胶，且两组分均为可交联大孔水凝胶，在制备用于细胞输送和组织再生的大孔水凝胶方面具有很大的应用潜力。
Macroporous hydrogels have been attracting increasingly attention in cell delivery and tissue regeneration. Assembling microgels is an effective method for preparing macroporous hydrogels. However, current methods are limited to specific requirements to devices and hydrogel inherent properties. In addition, most of macroporous hydrogels lack self-healing abilities due to the permanent covalent crosslink of microgels. In this study, we proposed an effective strategy for preparing a bioactive macroporous self-healable hydrogel by producing microgels through squeezing methacrylated hyaluronic acid (MeHA) and 3-aminophenylboronic acid modified sodium alginate (SABA) nanoporous hydrogels through steel meshes and assembling the MeHA-SABA microgels under alkaline condition created by bioglass (BG) ionic products. Results demonstrated that the obtained macroporous hydrogels have significant larger pore size than nanoporous hydrogels, which facilitated cell infiltration, viability and proliferation. Meanwhile, the macroporous hydrogels had good self-healing abilities with the formation of dynamic B-O bonds. Furthermore, with the bioactivity of BG, the hydrogels could induce cell migration and ingrowth of cells and blood vessels. Thus, bioactivity and macroporous structure of the hydrogels can work synergistically to stimulate tissue regeneration. Taken together, this strategy is effective, simple and can be widely applied in fabricating binary components self-healable macroporous hydrogels as long as the two components are crosslinkable, indicating a great application potential in fabricating macroporous hydrogels for cell delivery and tissue regeneration.