CYCLIC ADENOSINE 3',5'-MONOPHOSPHATE CONCENTRATION IN PANCREAS FOLLOWING STIMULATION BY SECRETIN, CHOLECYSTOKININ-PANCREOZYMIN AND ACETYLCHOLINE
CYCLIC ADENOSINE 3',5'-MONOPHOSPHATE CONCENTRATION IN PANCREAS FOLLOWING STIMULATION BY SECRETIN, CHOLECYSTOKININ-PANCREOZYMIN AND ACETYLCHOLINE
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DOI：
10.1113/jphysiol.1972.sp009868
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发表时间：
1972-01-01
影响因子：
5.5
通讯作者：
JOHNSON, M
中科院分区：
文献类型：
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作者：
CASE, RM;SCRATCHERD, T;JOHNSON, M
1.猫静脉注射促胰液素后，胰腺cAMP浓度在30秒内升高，1分钟内接近最大值。胰腺分泌仅在45秒后开始。随着分泌量的下降，环磷酸腺苷的浓度也随之下降。但在40分钟后，当分泌停止时，浓度再次上升，约80分钟后达到最大值，并在140分钟内恢复到基础值。在输注促胰液素的过程中，循环AMP的变化规律是相同的，但只要输注促胰液素，就能维持最初的升高。胰酶或乙酰胆碱单独或叠加促分泌素刺激后，环磷酸腺苷浓度也有类似的变化。然而，最初的升高只持续了30秒，在1分钟内接近基础浓度，并伴随着酶的分泌。在没有酶分泌的情况下，环磷酸腺苷的浓度随后又上升又下降，与分泌素刺激后的情况完全一样。使用隔离的、盐水灌流的猫胰腺制剂也进行了类似的观察。通过使用极低剂量的胰酶，可以在没有酶分泌的情况下观察到环磷酸腺苷浓度的第一次上升。同样，阿托品虽然阻断了酶的分泌，但不影响乙酰胆碱后环磷酸腺苷浓度的升高。浓度的第二次增加与分泌无关(可能与腺体合成可输出的酶有关)。虽然这些观察表明，环状AMP可能参与胰腺对分泌素、胰酶和乙酰胆碱的反应，但环状AMP浓度和分泌之间并不存在简单的关系。
1. Following anI.V.injection of secretin into anaesthetized cats, the pancreatic cyclic AMP concentration rose within 30 sec and reached near‐maximal values within 1 min. Pancreatic secretion began only after 45 sec. As secretion declined, the cyclic AMP concentration also fell. However, after 40 min, when secretion had ceased, the concentration again rose, reaching a maximum after about 80 min and returned to basal values within 140 min.2. During secretin infusion the pattern of cyclic AMP changes was the same, except that the initial rise was maintained as long as secretin was infused.3. Following either pancreozymin or acetylcholine, alone or super‐imposed on secretin stimulation, similar changes in cyclic AMP concentration were observed. However, the initial rise lasted only 30 sec, basal concentrations being approached within 1 min, and was accompanied by enzyme secretion. The concentration of cyclic AMP subsequently rose and fell again, in the absence of enzyme secretion, exactly as after secretin stimulation.4. Similar observations were made using an isolated, saline‐perfused preparation of the cat's pancreas.5. By using very low doses of pancreozymin it was possible to observe the first rise in cyclic AMP concentration in the absence of enzyme secretion. Similarly atropine, while blocking enzyme secretion, did not affect the rise in cyclic AMP concentration after acetylcholine. The second increase in concentration was never associated with secretion (it may have been connected with the synthesis of exportable enzymes by the gland).6. While these observations suggest that cyclic AMP may be involved in the response of the pancreas to secretin, pancreozymin and acetylcholine, no simple relation exists between cyclic AMP concentration and secretion.