Cytokine pattern in blister fluid and sera of patients with pemphigus
Cytokine pattern in blister fluid and sera of patients with pemphigus
复制标题
DOI：
10.1159/000063895
复制
发表时间：
2002-01-01
期刊：
影响因子：
3.4
通讯作者：
Ruocco, V
中科院分区：
文献类型：
--
作者：
Baroni, A;Perfetto, B;Ruocco, V
背景天疱疮（Pemphigus）是一种慢性自身免疫性水疱性疾病，主要有四种类型，即寻常型天疱疮（pemphigus vulgaris，PV）、落叶型天疱疮（pemphigus foliaceus，PF）、红斑型天疱疮（pemphigus erythematosus，PE）和增殖型天疱疮（pemphigus vegetans）。其共同的组织学特征是棘层松解。目的：以大疱性类天疱疮（BP）和大疱性接触性皮炎（BCD）患者的疱液为对照，比较天疱疮患者疱液和血清中某些细胞因子的水平。研究方法：采用免疫酶联免疫吸附试验（ELISA），我们测试了16名血清和6个水疱液的各种形式的天疱疮患者（13与PV，1与PF，2与PE），16名健康对照组的血清，5个水疱液的BP患者和5个水疱液的BCD患者，一些细胞因子（IL-10，IL-8和IFN-γ）的存在。细胞间抗体进行了搜索和滴定，桥粒芯糖蛋白1和3抗体水平进行了独立评估，比较他们的皮肤和口腔受累的严重程度。结果：天疱疮患者血清中IL-10水平低于检测限。与对照组相比，IL-8仅在天疱疮患者血清的4个样品中显著增加，而与对照组血清相比，在几乎所有患者中检测到低水平的IFN-γ。天疱疮患者疱液中细胞因子水平明显高于血清。BP和BCD患者疱液中细胞因子的表达与天疱疮患者相比存在差异。结论：本报告讨论了IL-10在天疱疮的慢性形式中发挥的抗炎作用，以及IL-8在中性粒细胞和淋巴细胞-单核细胞募集中可能发挥的作用的假设。版权所有（C）2002 S. Karger AG，巴塞尔。
Background. Pemphigus is a chronic auto-immune blistering disease with four main variants, i.e. pemphigus vulgaris (PV), foliaceus (PF), erythematosus (PE) and vegetans. The common histological feature of this disease is acantholysis. Objective:The aim of this study was to compare levels of some cytokines in blister fluid and sera of patients with pemphigus, using as control blister fluid of patients with bullous pemphigoid (BP) and bullous contact dermatitis (BCD). Methods: Using an immuno-enzymatic assay (ELISA), we tested 16 sera and 6 blister fluids of patients with various forms of pemphigus (13 with PV, 1 with PF, 2 with PE), the sera of 16 healthy control subjects, 5 blister fluids of patients with BP and 5 blister fluids of patients with BCD, for the presence of some cytokines (IL-10, IL-8 and IFN-gamma). Intercellular antibodies were searched for and titred; desmoglein 1 and 3 antibody levels were independently evaluated to compare them with the severity of both cutaneous and oral involvement. Results: The levels of IL-10 in the sera of patients with pemphigus were below the detection limits. IL-8 was significantly increased only in 4 samples of sera from pemphigus patients compared with controls, while IFN-gamma was detected at low levels in almost all patients compared with sera of controls. The cytokine levels in blister fluid of patients with pemphigus were significantly higher than in the sera. There was a difference between the expression of cytokines in blister fluid of control patients with BP and BCD compared with those of pemphigus patients. Conclusion: This report discusses the anti-inflammatory role played by IL-10 in the chronic form of pemphigus and the hypothesis of a possible role of IL-8 in neutrophil and lymphocyte-monocyte recruitment. Copyright (C) 2002 S. Karger AG, Basel.