Measurement of blood acetoacetate and beta-hydroxybutyrate in an automatic analyser.
Measurement of blood acetoacetate and beta-hydroxybutyrate in an automatic analyser.
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DOI：
10.1155/s1463924601000086
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发表时间：
2001
影响因子：
--
通讯作者：
Jimenez O
中科院分区：
文献类型：
--
作者：
Galán A;Hernández J;Jimenez O
β-羟基丁酸和乙酰乙酸以及乳酸和丙酮酸是通常存在于血液中的中间代谢物。g-羟基丁酸/乙酰乙酸比率是线粒体氧化还原状态的表达。该酮体比率可为电子传递链和丙酮酸代谢先天性缺陷的诊断和代谢状态提供线索。这些分析方法的标准化改善了对结果的解释，有助于儿童线粒体疾病的诊断。本研究描述了对Dimension R 2 L（Dade Behring，纽瓦克，特拉华州，美国）自动分析仪的调整，用于测量血酮体（g-羟基丁酸酯和乙酰乙酸酯）的方法。该方法允许直接测量未脱蛋白血浆（氟化物/乙二胺四乙酸）中的代谢物。这种调整简化了分析程序，并将周转时间限制在20分钟内。使用200 μ l的样品体积，可以测量12 - 1300 μ molL−1 g-羟基丁酸和10 - 450 μ molL−1乙酰乙酸的代谢物浓度，并获得可靠的分析响应。
g-hydroxybutyrate and acetoacetate as well as lactate and pyruvate are intermediary metabolites normally present in blood. The g-hydroxybutyrate/acetoacetate ratio is an expression of the mitochondrial oxido-reduction state. This ketone body ratio can provide a clue to diagnosis and metabolic status in congenital errors of the electron transport chain and pyruvate metabolism. The standardization of these analytical procedures improves the interpretation of the results helping in the difficult diagnosis of mitochondrial diseases in children. This study describes an adaptation to a Dimension R 2 L (Dade Behring, Newark, Delaware, USA) automatic analyser for a method to measure blood ketone bodies (g-hydroxybutyrate and acetoacetate). The method allows the metabolites to be measured directly in nondeproteinized plasma (fluoride/ethylenediaminetetraacetic acid). This adaptation simplifies the analytical procedure and limits the turnaround time to 20 minutes. With a sample volume of 200 μ l metabolite concentrations ranging from 12 to 1300 μ molL−1 of g-hydroxybutyrate and from 10 to 450 μ molL−1 of acetoacetate may be measured with a reliable analytical response.