Toxicity of CuO nanoparticles and Cu ions to tight epithelial cells from Xenopus laevis (A6): effects on proliferation, cell cycle progression and cell death.
Toxicity of CuO nanoparticles and Cu ions to tight epithelial cells from Xenopus laevis (A6): effects on proliferation, cell cycle progression and cell death.
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DOI：
10.1016/j.tiv.2012.12.013
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发表时间：
2013-08
期刊：
影响因子：
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通讯作者：
Amalie Thit;H. Selck;H. Bjerregaard
中科院分区：
文献类型：
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作者：
Amalie Thit;H. Selck;H. Bjerregaard
纳米粒子（NPs）由于其小尺寸（1- 100 nm）和高表面积体积比而具有独特的化学和物理性质。这意味着NP比它们的散装对应物潜在地更具毒性。在本研究中，使用来自非洲爪蟾（Xenopus laevis）的培养上皮细胞系（A6）来研究3种不同形式的铜（Cu）的毒性; Cu离子（Cu 2+）、CuO NPs（6 nm）和多分散的CuO NPs（100 nm，poly-CuO）。浓度为143-200μM的连续暴露表明，3种供试Cu形式的细胞毒性不同，且效应取决于细胞状态（分裂或分化）。用poly-CuO、CuONPs（6 nm）或Cu 2+处理的分裂细胞分别在处理48 h、6和7天后显示细胞周期停滞，并通过凋亡引起细胞死亡显著增加。用CuO NPs（6 nm）或Cu 2+处理引起细胞增殖的显著降低。分化细胞的治疗，揭示了相同的模式的毒性铜形式测试，但在较短的曝光时间。
Nanoparticles (NPs) have unique chemical and physical properties caused by their small size (1–100nm) and high surface to volume ratio. This means that the NPs are potentially more toxic than their bulk counterparts. In the present study a cultured epithelial cell line from Xenopus laevis (A6) was used to investigate toxicity of copper (Cu) in 3 different forms; Cu ions (Cu2+), CuO NPs (6nm) and poly-dispersed CuO NPs (100nm, poly-CuO). Continuous exposures at concentrations of 143–200μM demonstrated that cytotoxicity differed among the 3 Cu forms tested and that the effects depend on cell state (dividing or differentiated). Dividing cells treated with poly-CuO, CuO NPs (6nm) or Cu2+showed cell cycle arrest and caused significant increase in cell death via apoptosis after 48h, 6 and 7days of treatment, respectively. Treatment with either CuO NPs (6nm) or Cu2+caused significant decrease in cell proliferation. Treatments of differentiated cells, revealed the same patterns of toxicity for Cu forms tested, but after shorter exposure periods.