A variant of estrogen receptor-α, ER-α36 is expressed in human gastric cancer and is highly correlated with lymph node metastasis
A variant of estrogen receptor-α, ER-α36 is expressed in human gastric cancer and is highly correlated with lymph node metastasis
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DOI：
10.3892/or_00000842
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发表时间：
2010-07-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Lijiang
中科院分区：
文献类型：
--
作者：
Deng, Hao;Huang, Xuan;Liu, Lijiang
胃癌是世界上最常见的癌症之一；近三分之二的胃癌病例和死亡发生在欠发达地区。胃癌发生过程中的分子和细胞事件仍不清楚。在此，我们检测了 ER-α 36（一种 ER-α 变体）在已建立的胃癌细胞系和 22 名胃癌患者标本中的表达。采用RT-PCR、Western blot和免疫组织化学方法评估ER-α 36的表达水平。通过免疫荧光测定确定ER-α 36在胃癌细胞中的定位。在所有已建立的胃癌细胞系中均检测到 ER-α 36 的 mRNA 和蛋白质。与配对的正常组织相比，在检查的 22 个肿瘤样本中，有 17 个 (77.27%) 表达了更高水平的 ER-α 36 mRNA (p
Gastric cancer is one of the most common cancers in the world; almost two-thirds of gastric cancer cases and deaths occur in less developed regions. The molecular and cellular events during development of gastric cancer remain unclear. Herein, we examined the expression of ER-alpha 36, an ER-alpha variant, in established gastric cancer cell lines and specimens from 22 gastric cancer patients. RT-PCR, Western blot and immunohistochemistry methods were used to assess expression levels of ER-alpha 36. ER-alpha 36 localization in gastric cancer cells was determined with an immunofluorenscence assay. Both mRNA and protein of ER-alpha 36 were detected in all established gastric cancer cell lines examined. Higher levels of ER-alpha 36 mRNA were expressed in 17 of 22 (77.27%) tumor specimens examined compared to the paired normal tissues (p