Loss of the zona pellucida in mice, and the effect of tubal ligation and ovariectomy.
Loss of the zona pellucida in mice, and the effect of tubal ligation and ovariectomy.
复制标题
小鼠透明带的丧失以及输卵管结扎和卵巢切除术的影响。
DOI：
10.1530/jrf.0.0130485
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
A. McLaren
中科院分区：
文献类型：
--
作者：
M. W. Orsini;A. McLaren
总结。小鼠的透明带在妊娠第4天晚些时候开始丧失。到23.00小时，大约一半的囊胚是去透明带的。桃金娘蓝敏感度，表明依恋的第一阶段，大约在同一时间开始，或者稍晚一点。一旦透明带丢失，囊胚的恢复就变得越来越困难。然而，由于早期的附着非常肤浅，一些无透明带的胚泡可以被冲出子宫，直到怀孕第5天晚上。蜕膜反应发生在第5天中期。当卵子通过结扎保留在输卵管中时，透明带的丧失延迟了大约12小时，而当怀孕早期进行卵巢切除时，透明带的丢失延迟了更长的时间。在这样的雌性动物中，在子宫中没有观察到潘塔明蓝反应。在这些实验条件下，最终会发生卵带断裂，可能是由于胚泡部分的主动脉动运动；胚泡可能会在破裂的卵带内停留一段时间，也可能会出现。在正常的雌激素刺激的子宫中，卵泡带的丢失似乎也是通过孵化来实现的，但此外，一些由激素决定的子宫因子可能会作用于卵泡带以削弱它，从而加速囊胚的出现。
Summary. Loss of the zona pellucida begins late on the 4th day of gestation in the mouse. By 23.00 hours, about half the blastocysts are zona-free. Pontamine Blue sensitivity, indicating the first stage of attachment, begins at about the same time, or a little later. Once the zona is lost, recovery of blastocysts becomes increasingly difficult. However, because attachment in the early stages is very superficial, some zona-free blastocysts can be flushed out of the uterus up to the evening of the 5th day of gestation. The decidual reaction develops during the middle of the 5th day. Loss of the zona pellucida is delayed by about 12 hr when the ova are retained in the oviducts by ligation, and for a longer period when ovariectomy is performed early in pregnancy. In such females, no Pontamine Blue reaction is observed in the uterus. Under these experi-mental conditions, fracture of the zona eventually occurs, probably owing to active pulsating movements on the part of the blastocyst; the blastocyst may remain for a time within the ruptured zona, or may emerge. In a normal, oestrogen-stimulated uterus, loss of the zona also seems to be by hatching, but in addition some hormonally-determined uterine factor is probably acting on the zona to weaken it, and thus accelerate the process of emergence of the blastocyst.