Purification and some characteristics of two human serum proteins inhibiting papain and other thiol proteinases
Purification and some characteristics of two human serum proteins inhibiting papain and other thiol proteinases
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两种抑制木瓜蛋白酶和其他硫醇蛋白酶的人血清蛋白的纯化及一些特性
DOI：
10.1016/0014-5793(79)80588-8
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发表时间：
1979
期刊：
影响因子：
3.5
通讯作者：
M. Järvinen
中科院分区：
文献类型：
--
作者：
M. Järvinen
人血清含有7种充分表征的蛋白酶抑制剂，对丝氨酸蛋白酶具有或多或少的广泛特异性[1，2]。此外，已经描述了两种不抑制丝氨酸蛋白酶的抑制剂：fl，-胶原酶抑制剂[3]和cYz-巯基蛋白酶抑制剂[4]。部分纯化的cuz-thiol蛋白酶抑制剂已被证明与已知的丝氨酸蛋白酶抑制剂不同。它的性质也与我们实验室纯化的人表皮巯基蛋白酶抑制剂的性质完全不同[5，6]。纯化的抑制剂在其免疫学性质和抑制谱方面彼此相似，但具有不同的电荷和分子大小。具有cYz-迁移率的抑制剂似乎与[4]中的巯基蛋白酶抑制剂相同。这项工作的初步说明已经发表[7]。
Human serum contains 7 well characterized proteinase inhibitors with more or less broad specificity to serine proteinases [1, 2]. In addition, two inhibitors not inhibiting serine proteinases have been described: fl,-collagenase inhibitor [3] and cYz-thiol proteinase inhibitor [4]. The partially purified cuz-thiol proteinase inhibitor has been shown not to be identical with the known inhibitors of serine proteinases. Its properties are also quite different from those of the human epidermal thiol proteinase inhibitor, purified in our laboratory [5, 6].Here I describe the purification of two thiol proteinase inhibitors with (ILL-and CQ-mobilities from human serum. The purified inhibitors resemble each other in their immunological properties and inhibiting spectra, but have different charges and molecular sizes. The inhibitor with cYz-mobility seems to be identical with the thiol proteinase inhibitor in [4]. A preliminary note of this work has been published [7].