Isolation and characterization of the HO gene from the yeast Saccharomyces paradoxus.
Isolation and characterization of the HO gene from the yeast Saccharomyces paradoxus.
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DOI：
10.1016/s1567-1356(03)00152-1
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发表时间：
2003-10
影响因子：
3.2
通讯作者：
T. Kodama;T. Hisatomi;K. Uchida;T. Yamaki;M. Tsuboi
中科院分区：
文献类型：
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作者：
T. Kodama;T. Hisatomi;K. Uchida;T. Yamaki;M. Tsuboi
从酵母中分离到一个与酿酒酵母同源(HO)基因同源的DNA片段，该片段含有一个开放阅读框，其编码序列与酿酒酵母HO基因的编码序列有90.9%的同源性。Cerevisiae HOgene.PutativeHO3基因在异源单倍体菌株中诱导二倍体。酿酒。系统发育分析表明，Saccharmycessensu的5个严格的HOO基因的编码区和5‘-上游调控区是共同进化的。悖论在系统发育上更接近于S。酿酒而不是酿酒。巴亚努斯。最后，从原始的同源同源类型菌株OFS中分离到异源单倍体菌株。扰乱这些自相矛盾的东西。矛盾的HOGIGN与这些。酿酒酵母URA3基因。
A DNA fragment homologous to the homothallism (HO) gene ofSaccharomyces cerevisiaewas isolated fromSaccharomyces paradoxusand was found to contain an open reading frame that was 90.9% identical to the coding sequence of theS. cerevisiae HOgene. The putativeHOgene was shown to induce diploidization in a heterothallic haploid strain fromS. cerevisiae. Phylogenetic analysis revealed that the coding and 5′-upstream regulatory regions from fiveSaccharomycessensu strictoHOgenes have coevolved, and thatS. paradoxusis phylogenetically closer toS. cerevisiaethan toS. bayanus. Finally, heterothallic haploid strains were isolated from the original homothallic type strain ofS. paradoxusby disrupting theS. paradoxus HOgene with theS. cerevisiae URA3gene.