Mutations in the Anopheles gambiae pink-eye and white genes define distinct, tightly linked eye-color loci
Mutations in the Anopheles gambiae pink-eye and white genes define distinct, tightly linked eye-color loci
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DOI：
10.1093/oxfordjournals.jhered.a022952
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发表时间：
1996-01-01
影响因子：
3.1
通讯作者：
Collins, FH
中科院分区：
文献类型：
--
作者：
Benedict, MQ;Besansky, NJ;Collins, FH
通过 EMS 或伽马射线照射，在冈比亚按蚊中诱导了新的眼睛颜色突变，分离出 7 个新的性连锁突变，其中 5 个是可存活且完全可育的，其中 3 个位于先前描述的红眼 (p) 基因中，其中先前已鉴定出两个自发突变，另外两个突变 w(1) 和 w(2) 位于没有现存突变等位基因的基因中，我们将其指定为白色基因，其中之一， w(1) 是由于黑腹果蝇白色基因克隆同源物 5' 端的大缺失，红眼和白色基因座与 w(1) 或 w(2) 和自发突变等位基因 p(w) 之间的重组频率紧密连锁，分别为 3.5% 和 1.1%。 对 F-2 幼虫的小样本进行了各种等位基因之间基因内重组的检查，但在任何实验中均未观察到，白色突变体（但不是红眼病）在幼虫体色素沉着表型无领（+）以及雄性附属腺和睾丸鞘的色素沉着的表达上表现出上位性。这些多效性效应与黑腹果蝇白色突变体相似，并且还表明白色可能与先前描述的白眼基因相同。
New eye-color mutations were induced in the mosquito Anopheles gambiae by EMS or gamma-irradiation treatments, Seven new sex-linked mutations were isolated, five of which were viable and fully fertile, Of those, three were in the previously described pink-eye (p) gene in which two spontaneous mutations have previously been identified, Two other mutations, w(1) and w(2), were in a gene with no extant mutant alleles that we designate the white gene, One of these, w(1), is due to a large deletion in the 5' end of the cloned homolog of the D. melanogaster white gene, The pink-eye and white loci are tightly linked with recombination frequencies of 3.5% and 1.1% between w(1) or w(2) and the spontaneous mutant allele, p(w), respectively, Small samples of F-2 larvae were examined for intragenic recombination between various alleles, but none was observed in any experiment, The white mutants, but not the pink-eye, exhibit epistasis over the expression of the larval body pigmentation phenotype collarless(+) and pigmentation of the male accessory glands and testis sheath, These pleiotropic effects are similar to those of D. melanogaster white mutants and also suggest that white is probably identical to the previously described white-eye gene.