Twenty-Eight-Day Repeated Inhalation Toxicity Study of Aluminum Oxide Nanoparticles in Male Sprague-Dawley Rats.
Twenty-Eight-Day Repeated Inhalation Toxicity Study of Aluminum Oxide Nanoparticles in Male Sprague-Dawley Rats.
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DOI：
10.5487/tr.2018.34.3.343
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发表时间：
2018-10
影响因子：
2.3
通讯作者：
Lim CH
中科院分区：
文献类型：
--
作者：
Kim YS;Chung YH;Seo DS;Choi HS;Lim CH
氧化铝纳米颗粒（Al 2 O3 NPs）是使用最广泛的纳米材料之一;然而，关于其风险识别和评估的信息相对较少。在本研究中，我们的目的是调查的潜在毒性的Al 2 O3纳米粒子反复吸入暴露在雄性Sprague-Dawley大鼠。大鼠暴露于Al 2 O3纳米颗粒28天（5天/周），剂量为0，0.2，1，和5毫克/立方米，使用鼻吸入系统。在实验期间，我们评价了临床体征、体重变化、血液学和血清生化参数、尸检结果、器官重量和组织病理学结果。此外，我们分析了支气管肺泡灌洗液（BALF），包括白细胞分类计数，以及主要器官和血液中的铝含量。暴露组肺组织中铝含量最高，并呈剂量依赖关系。组织学显示，在接触和恢复期间，5 mg/m3剂量组大鼠的肺中出现肺泡巨噬细胞积聚。这些变化在恢复期结束时趋于增加。在BALF分析中，暴露期间，1和5 mg/m3组的总细胞和中性粒细胞计数以及乳酸脱氢酶、肿瘤坏死因子-α和白细胞介素-6水平显著升高。在本实验条件下，我们建议Al_2O_3纳米颗粒对雄性大鼠的无明显不良反应水平为1 mg/m3，靶器官为肺。
Aluminum oxide nanoparticles (Al2O3 NPs) are among the most widely used nanomaterials; however, relatively little information about their risk identification and assessment is available. In the present study, we aimed to investigate the potential toxicity of Al2O3 NPs following repeated inhalation exposure in male Sprague-Dawley rats. Rats were exposed to Al2O3 NPs for 28 days (5 days/week) at doses of 0, 0.2, 1, and 5 mg/m3 using a nose-only inhalation system. During the experimental period, we evaluated the clinical signs, body weight change, hematological and serum biochemical parameters, necropsy findings, organ weight, and histopathology findings. Additionally, we analyzed the bronchoalveolar lavage fluid (BALF), including differential leukocyte counts, and aluminum contents in the major organs and blood. Aluminum contents were the highest in lung tissues and showed a dose-dependent relationship in the exposure group. Histopathology showed alveolar macrophage accumulation in the lungs of rats in the 5 mg/m3 group during exposure and recovery. These changes tended to increase at the end of the recovery period. In the BALF analysis, total cell and neutrophil counts and lactate dehydrogenase, tumor necrosis factor-α, and interleukin-6 levels significantly increased in the 1 and 5 mg/m3 groups during exposure. Under the present experimental conditions, we suggested that the no-observed-adverse-effect level of Al2O3 NPs in male rats was 1 mg/m3, and the target organ was the lung.