Micropatterned substrates for the growth of functional neuronal networks of defined geometry
Micropatterned substrates for the growth of functional neuronal networks of defined geometry
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DOI：
10.1021/bp034016f
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发表时间：
2003-09-01
影响因子：
2.9
通讯作者：
Offenhäusser, A
中科院分区：
文献类型：
--
作者：
Vogt, AK;Lauer, L;Offenhäusser, A
控制细胞位置和连接性的神经元网络的体外组装是一种令人着迷的方法，不仅适用于基础神经科学研究的主题，而且适用于生物传感器和组织工程等多种应用。我们在微接触印刷创建的图案基底上培养大鼠胚胎皮质神经元。使用聚苯乙烯作为细胞排斥背景，在其上应用生理蛋白质的网格图案。我们打印了层粘连蛋白和细胞外基质蛋白的混合物，另外这两个系统都与聚赖氨酸混合。在所有四种情况下都可以观察到细胞以高保真度附着到图案上，以及图案上相邻细胞之间化学突触的形成，但在含有聚赖氨酸的样品中细胞附着强烈增加。在图案化基底上生长的神经元的膜容量小于均匀涂层对照上的神经元的膜容量，我们将其归因于几何限制，但在静息膜电位或它们形成的突触质量方面没有差异。因此，我们相信细胞在模式上正常附着和分化，并按照预定义的几何形状形成功能性的、成熟的突触。
The in vitro assembly of neuronal networks with control over cell position and connectivity is a fascinating approach not only for topics in basic neuroscience research but also in diverse applications such as biosensors and tissue engineering. We grew rat embryonic cortical neurons on patterned substrates created by microcontact printing. Polystyrene was used as a cell repellent background, onto which a grid pattern of physiological proteins was applied. We printed laminin and a mixture of extracellular matrix proteins and additionally both systems mixed with polylysine. Attachment of cells to the pattern with high fidelity as well as the formation of chemical synapses between neighboring cells on the pattern could be observed in all four cases, but cell attachment was strongly increased on samples containing polylysine. Neurons grown on patterned substrates had a membrane capacity smaller than that of neurons on homogeneously coated controls, which we attributed to the geometrical restrictions, but did not differ either in resting membrane potential or in the quality of synapses they formed. We therefore believe that the cells attach and differentiate normally on the pattern and form functional, mature synapses following the predefined geometry.