Delivery of antigen to sialoadhesin or CD163 improves the specific immune response in pigs
Delivery of antigen to sialoadhesin or CD163 improves the specific immune response in pigs
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DOI：
10.1016/j.vaccine.2011.04.076
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发表时间：
2011-06-24
期刊：
影响因子：
5.5
通讯作者：
Revilla, Concepcion
中科院分区：
文献类型：
--
作者：
Poderoso, Teresa;Martinez, Paloma;Revilla, Concepcion
将抗原递送至抗原呈递细胞表面受体代表了改善对弱免疫原性抗原的免疫应答的有前景的策略。我们分析了猪唾液酸粘附素（Sn）和CD 163作为抗原靶向受体的潜力，使用小鼠Ig作为替代抗原。Sn和CD 163是两种内吞受体，主要表达于位于次级淋巴器官抗原取样区的巨噬细胞上。当使用来自用小鼠血清IgG免疫的猪的细胞作为应答细胞时，抗CD 163的MAb在比对照mAb低50-80倍的浓度下诱导体外PBMC增殖。为了评估体内靶向，将猪皮下免疫。用抗Sn、抗CD 163或对照mAb，并分析对小鼠IG诱导的免疫应答。第一次免疫后两周，接受抗Sn或抗CD 163 mAb的猪开始显示出比对照更高的抗小鼠IgG血清滴度。6周后加强，进一步增加抗IgG滴度高达对照的15-60倍。此外，观察到应答中IgG 1或IgG 2亚类的相对优势存在差异，这取决于Sn或CD 163靶向。用抗Sn mAb免疫的猪的外周血单个核细胞对小鼠IgG的增殖反应比用对照mAb免疫的猪的细胞高。这些结果表明，靶向抗原Sn或CD 163可以增强猪的免疫应答。(C)2011爱思唯尔有限公司保留所有权利。
Delivery of antigens to antigen presenting cell surface receptors represents a promising strategy to improve immune response to weak immunogenic antigens. We have analyzed the potential of porcine sialoadhesin (Sn) and CD163 as antigen targeting receptors using mouse Igs as surrogate antigens. Sn and CD163 are two endocytic receptors mainly expressed on macrophages located in antigen-sampling zones of secondary lymphoid organs. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs. To evaluate in vivo targeting, pigs were immunized s.c. with anti-Sn, anti-CD163 or control mAbs, and the immune response induced to mouse Ig was analyzed. Two weeks after the first immunization, pigs receiving either anti-Sn or anti-CD163 mAbs started to show higher anti-mouse-IgG serum titres than controls. Boosting 6 weeks later, further increased the anti-IgG titres up to 15-60 fold those of controls. In addition, differences in the relative predominance of IgG1 or IgG2 subclasses in the response depending on Sn or CD163 targeting were observed. Peripheral blood mononuclear cells from pigs immunized with anti-Sn mAb showed a higher proliferative response to mouse IgG than cells from pigs immunized with control mAb. These results show that targeting antigen to Sn or CD163 can enhance the immune response in pigs. (C) 2011 Elsevier Ltd. All rights reserved.