Deprivation of endogenous brain-derived neurotrophic factor results in impairment of spatial learning and memory in adult rats
Deprivation of endogenous brain-derived neurotrophic factor results in impairment of spatial learning and memory in adult rats
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DOI：
10.1016/s0006-8993(99)01592-9
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发表时间：
1999-07-24
期刊：
影响因子：
2.9
通讯作者：
Zhou, XF
中科院分区：
文献类型：
--
作者：
Mu, JS;Li, WP;Zhou, XF
脑源性神经营养因子(BDNF)在大脑皮质和海马区大量表达，参与突触可塑性和长时程增强(LTP)。本研究旨在探讨内源性脑源性神经营养因子在大鼠空间学习记忆中的作用。用渗透泵将抗BDNF抗体(n=7)或对照免疫球蛋白G(n=6)连续注入大鼠脑内7d。然后，这些老鼠接受了一系列的行为测试。结果表明，脑源性神经营养因子抗体治疗组小鼠的平均逃避潜伏期明显长于对照组(F=13.3p<；0.001)。与其他三个象限(p<；0.05)相比，对照组大鼠在P象限(放置逃生平面的地方)游泳的距离明显更长。相比之下，接受抗BDNF治疗的大鼠在所有四个象限游泳的距离相同。抗脑源性神经营养因子治疗组大鼠在P象限的平均游泳距离百分比明显低于对照组(P<；0.001)。这些结果表明，成年大鼠的空间学习和记忆需要内源性BDNF。(C)1999 Elsevier Science B.V.保留所有权利。
Brain-derived neurotrophic factor (BDNF) is abundantly expressed in the hippocampus and cerebral cortex and is involved in synaptic plasticity and long-term potentiation (LTP). The present study was under taken to investigate whether endogenous BDNF was required for spatial learning and memory in a rat model. Antibodies to BDNF(anti-BDNF, n = 7) or control immunoglobulin G (control, n = 6) were delivered into the rat brain continuously for 7 days with an osmotic pump. The rats were then subjected to a battery of behavioral tests. The results show that the average escape latencies in the BDNF antibody treated group were dramatically longer than those of the control (F = 13.3, p < 0.001). The rats treated with control IgG swam for a significantly longer distance in the P quadrant (where the escape plane had been placed) compared with the other three quadrants (p < 0.05). In contrast, anti-BDNF-treated rats swam an equivalent distance in all four quadrants. The average percentage of swimming distance in the P quadrant by anti-BDNF-treated rats was much less than that by control IgG treated rats (p < 0.001). These results suggest that endogenous BDNF is required for spatial learning and memory in adult rats. (C) 1999 Elsevier Science B.V. All rights reserved.