Strategy for data collection from protein crystals using a multiwire counter area detector diffractometer
Strategy for data collection from protein crystals using a multiwire counter area detector diffractometer
复制标题
使用多线计数器面积检测器衍射仪从蛋白质晶体收集数据的策略
DOI：
10.1107/s0021889885010433
复制
发表时间：
1985
影响因子：
6.1
通讯作者：
D. Anderson
中科院分区：
文献类型：
--
作者：
N. Xuong;C. Nielsen;R. Hamlin;D. Anderson
描述了一种利用配备多线正比计数器的衍射仪进行数据采集的策略。数据是使用电子旋转法收集的，这种方法类似于早先开发的“静止”方法。数据是以三到十二次或更多次的“运行”为一组收集的。数据收集“Run”由成百上千张电子旋转图片组成，每张图片的ω都提前了特定的量(根据晶体的马赛克程度，从0.07°到0.2°不等)。一次拍摄中的每一张照片都是以相同的方式拍摄的：χ和ϕ设置角度和曝光时间。关于如何从这些电子旋转图像中提取强度数据的信息在其他地方给出。这里，唯一关心的是如何选择一组ω，χ和ϕ角度来定义一组运行，以便在最短的时间内获得完整的数据集。首先讨论了衍射几何，然后概述了该策略，最后举例说明了该策略在一些典型的蛋白质数据采集问题中的应用。
A strategy for data collection using a diffractometer equipped with a multiwire proportional counter is described. Data are collected using an electronic rotation method similar to an earlier developed `still' method. Data are collected as a set of three to 12 or more `runs'. A data collection `run' consists of a consecutive series of hundreds of electronic rotation pictures with ω advanced by a specific amount (from 0.07 to 0.20°, depending on the mosaicity of the crystal) during each picture. Every picture in a run is taken at the same: χ and ϕ setting angles and exposure time. Information on how to extract the intensity data from these electronic rotation pictures is given elsewhere. Here, the only concern is how to choose a set of ω, χ and ϕ angles for defining a set of runs in order to get a complete set of data in a minimum time. First the diffraction geometry is discussed, then the strategy is outlined; finally, some examples are given to illustrate how the strategy is used in some typical protein data collection problems.