Localisation of the protein and glycoprotein components of bovine nasal epithelial desmosomes by immunoelectron microscopy.
Localisation of the protein and glycoprotein components of bovine nasal epithelial desmosomes by immunoelectron microscopy.
复制标题
通过免疫电子显微镜定位牛鼻上皮桥粒的蛋白质和糖蛋白成分。
作者：
K. Miller;D. Mattey;H. Measures;C. Hopkins;D. Garrod
通过免疫金标记牛鼻粘膜冰冻切片，发现桥粒蛋白（dp 1 - 4）和糖蛋白（dg 1 - 3）定位于牛鼻粘膜上皮的桥粒内。从细胞外间隙开始，通过斑块到张力细丝，发现了以下定位：标记为130，000和115，000 Mr的糖蛋白（dg 2和dg 3）主要位于细胞外间隙，该位置与其提出的粘附功能一致。175，000 - 164，000 Mr（dg 1）的糖蛋白也存在于细胞外空间，此外还具有延伸至整个胞质斑块的胞质结构域。83，000 Mr蛋白（dp 3）位于沿着膜的细胞质面并延伸到斑块中，而识别83，000 Mr蛋白（dp 3）和75，000 Mr蛋白（dp 4）的抗体在斑块内和斑块外都进行标记。高分子标记。Mr为250，000和215，000的wt蛋白（dp 1和dp 2）在很大程度上被排除在斑块之外，并且位于远端，邻近张力细丝。半桥粒不能用dg 1 - 3或dp 3和4的抗体标记，但用dp 1和2的抗体获得了一些标记。
Desmosomal proteins (dp1‐4) and glycoproteins (dg1‐3) have been localised within desmosomes of bovine nasal epithelium by immunogold labelling of ultrathin frozen sections. Beginning in the extracellular space and proceeding through the plaque to the tonofilaments, the following localisations were found. Labelling for the 130,000 and 115,000 Mr glycoproteins (dg2 and dg3) was predominantly in the extracellular space, a location consistent with their proposed adhesive function. The glycoproteins of 175,000‐164,000 Mr (dg1) were also found in the extracellular space and in addition had cytoplasmic domains extending throughout the cytoplasmic plaque. The 83,000 Mr protein (dp3) was located along the cytoplasmic face of the membrane and extended into the plaque, whereas an antibody which recognises both the 83,000 Mr protein (dp3) and the 75,000 Mr protein (dp4) gave labelling both in and beyond the plaque. Labelling for the high mol. wt proteins of Mr 250,000 and 215,000 (dp1 and dp2) was largely excluded from the plaque, and was located distally, adjacent to the tonofilaments. Hemidesmosomes could not be labelled with antibodies to dg1‐3 or dp3 and 4, but some labelling was obtained with antibody to dp1 and 2.