ORIENTATION AND MOTION OF AMPHIPHILIC SPIN LABELS IN MEMBRANES
ORIENTATION AND MOTION OF AMPHIPHILIC SPIN LABELS IN MEMBRANES
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DOI：
10.1073/pnas.64.1.20
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发表时间：
1969-01-01
影响因子：
11.1
通讯作者：
MCCONNEL.HM
中科院分区：
文献类型：
--
作者：
HUBBELL, WL;MCCONNEL.HM
12-酮硬脂酸、5-酮硬脂酸、5-酮三十二烷酸和 5α-雄甾烷-3-酮-17β-醇的 N-氧基-4','-二甲基恶唑烷衍生物的顺磁共振谱已在超声处理的磷脂分散体、美洲白鹤的步行腿神经纤维和通过流体动力剪切定向的红细胞中观察到。这些自旋标记在神经和红细胞膜中的优选方向是长两亲轴垂直于膜表面。共振光谱表明，神经纤维中的这些标记与脂质双层结合，与超声处理的磷脂分散体中发现的脂质双层非常相似，而红细胞中的任何脂质双层必须更加坚硬。
The paramagnetic resonance spectra of N-oxyl-4′,′-dimethyloxazolidine derivatives of 12-ketostearic acid, 5-ketostearic acid, 5-ketotricosanoic acid, and 5α-androstan-3-one-17β-ol have been observed in sonicated phospholipid dispersions, the walking-leg nerve fibers ofHomarus americanus, and in erythrocytes oriented by hydrodynamic shear. The preferred orientation of these spin labels in the nerve and erythrocyte membranes is one in which the long amphiphilic axis is perpendicular to the membrane surface. The resonance spectra indicate that these labels in the nerve fiber are bound to a lipid bilayer very similar to that found in the sonicated phospholipid dispersion, whereas any lipid bilayer in the erythrocyte must be much more rigid.