Mouse preferential incising force orientation changes during jaw closing muscle hyperalgesia and is sex dependent.
Mouse preferential incising force orientation changes during jaw closing muscle hyperalgesia and is sex dependent.
复制标题
小鼠优先切割力方向在下颌闭合肌肉痛觉过敏期间发生变化，并且是性别依赖性的。
DOI：
10.1016/j.physbeh.2016.09.005
复制
发表时间：
2016
影响因子：
2.9
通讯作者：
Morris-Wiman,J
中科院分区：
文献类型：
--
作者：
Widmer,CG;Morris-Wiman,J
小鼠切割是由一个中央模式发生器控制，这种活动可以改变疼痛的存在。切割频率和产生的最大力随着疼痛而降低。在这项研究中，我们使用重复的酸性注射在左咬肌的男性和女性小鼠，以确定基线和颌骨肌肉疼痛的条件和性别的影响之间的差异上的优惠切割directions.MethodsA内的主题设计被用来评估以前获得的数据使用多轴力数据（X、Y和Z）从每种性别的每只小鼠的第4个基线记录日和注射后第7天（最大疼痛反应日）。共评价了34只雌性和雄性（3-9月龄）CD-1小鼠。在数学旋转X和Y轴以使Y轴平行于融合器顶部的金属丝支柱后，分析数据以确定基线（无疼痛）和疼痛（第7天）条件下以及性别之间的切割方向偏好。X-Y、X-Z和Y-Z轴的雷达图描绘了每种性别在基线和疼痛条件之间的切割偏好的平均方向。组间的统计差异进行了测试，使用混合模型AN0VA.ResultsSimilarly以前的研究结果，雌性小鼠有一个更强大的差异切割方向偏好比较男性和女性的疼痛条件时，这是最明显的X-Z轴。最常受影响的切割频率为5.3、6.2和7.6 Hz。雄性小鼠在基线和疼痛条件之间的切割方向偏好变化不大。此外，对每个切割频率在Z轴和X轴上切割所用时间百分比的统计比较发现，当比较5.3和7.6 Hz频率时，切割偏好没有差异。最后，雌性小鼠使用了一种新的方法，以尽量减少疼痛，而切割旋转他们的头和身体近180度，而男性没有使用这种策略频繁.ConclusionsThe首选切割方向的变化在下颌肌肉疼痛的条件下，这个信息可以用来进一步表征功能性疼痛的咀嚼肌系统。这些变化取决于中央模式发生器产生的切割频率。
IntroductionMouse incising is controlled by a central pattern generator and this activity can change in the presence of pain. The incising frequency and maximum force generation decreases with pain. In this study, we used repetitive acidic injections in the left masseter muscle of male and female mice to determine differences between baseline and jaw muscle pain conditions and the effect of sex on preferential incising direction.MethodsA within subject design was used to evaluate data previously acquired using multi-axis force data (X, Y and Z) from the 4th baseline recording day and day 7 post-injection (day of maximal pain response) for each mouse of each sex. A total of 34 female and male (age 3–9 months) CD-1 mice were evaluated. After mathematically rotating the X and Y axes to align the Y axis to be parallel to the wire struts of the cage top, data were analyzed to determine incising direction preference during baseline (non-pain) and pain (day 7) conditions and between sex. Radar plots of X-Y, X-Z and Y-Z axes depicted the average direction of incising preference between baseline and pain conditions for each sex. Statistical differences among groups were tested using a mixed model ANOVA.ResultsSimilar to previous findings, female mice had a more robust difference in incising direction preference when comparing male and female pain conditions and this was most evident in the X-Z axes. The incising frequencies most commonly affected were 5.3, 6.2 and 7.6 Hz. Male mice varied little in their incising direction preference between the baseline and pain conditions. In addition, statistical comparison of ratios of the percent of time spent incising in the Z versus X axes for each incising frequency found that the incising preference was not different when comparing 5.3 and 7.6 Hz frequencies. Finally, female mice used a novel approach to minimize pain while incising by rotating their head and body nearly 180 degrees while males did not use this strategy as frequently.ConclusionsThe preferred incising direction changes in a jaw muscle pain condition and this information can be used to further characterize functional pain in the masticatory muscle system. The changes were dependent on the incising frequency generated by the central pattern generator for incising.