Introducing dielectrophoresis as a new force field for field-flow fractionation
Introducing dielectrophoresis as a new force field for field-flow fractionation
复制标题
DOI：
10.1016/s0006-3495(97)78144-x
复制
发表时间：
1997-08-01
影响因子：
3.4
通讯作者：
Gascoyne, PRC
中科院分区：
文献类型：
--
作者：
Huang, Y;Wang, XB;Gascoyne, PRC
我们介绍了介电泳场流分离的细胞表征和分离的原理，并给出了初步的实验结果。操作装置采用薄室的形式，其中底壁支撑微电极阵列。通过向这些电极施加适当的交流电压信号，产生介质电泳力以使悬浮在腔室中的细胞悬浮并影响它们的平衡高度，从而在腔室中建立层流行轮廓，从而使流体随着离腔壁距离的增加而流动得更快。在气流中携带的电池将达到平衡高度和相应的速度，这是基于它所经历的介电、重力和流体动力升力的平衡。我们描述了这个系统的理论模型，并证明了电池速度是平均流体速度、施加到电极上的信号的电压和频率的函数，最重要的是，电池的介电性质。以人白血病(HL-60)细胞平行电极阵列为例，验证了该模型的有效性，并将该装置应用于分离HL-60细胞和外周血单核细胞。
We present the principle of cell characterization and separation by dielectrophoretic field-flow fractionation and show preliminary experimental results. The operational device takes the form of a thin chamber in which the bottom wall supports an array of microelectrodes. By applying appropriate AC voltage signals to these electrodes, dielectrophoretic forces are generated to levitate cells suspended in the chamber and to affect their equilibrium heights, A laminar Row profile is established in the chamber so that fluid flows faster with increasing distance from the chamber walls. A cell carried in the flow stream will attain an equilibrium height, and a corresponding velocity, based on the balance of dielectrophoretic, gravitational, and hydrodynamic lift forces it experiences. We describe a theoretical model for this system and show that the cell velocity is a function of the mean fluid velocity, the voltage and frequency of the signals applied to the electrodes, and, most significantly, the cell dielectric properties. The validity of the model is demonstrated with human leukemia (HL-60) cells subjected to a parallel electrode array, and application of the device to separating HL-60 cells from peripheral blood mononuclear cells is shown.