Melatonin protects against maternal diabetes-associated meiotic defects by maintaining mitochondrial function.
Melatonin protects against maternal diabetes-associated meiotic defects by maintaining mitochondrial function.
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DOI：
10.1016/j.freeradbiomed.2022.06.243
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发表时间：
2022-07
影响因子：
7.4
通讯作者：
Xiaoqian Li;Yi Wang;Shujun Yang;Yu Liu;Xiang Ma;Lu Liu;Si-Hong Li;Dong Niu;Xing Duan
中科院分区：
文献类型：
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作者：
Xiaoqian Li;Yi Wang;Shujun Yang;Yu Liu;Xiang Ma;Lu Liu;Si-Hong Li;Dong Niu;Xing Duan
母亲糖尿病已被广泛报道对卵母细胞质量产生不利影响。虽然各种分子和途径可能参与了这一过程，但预防母体糖尿病诱导的卵母细胞质量恶化的策略仍未探索。褪黑激素是由松果体合成的，由于其抗氧化功能，已被证明对卵母细胞质量有有益的影响。在本研究中，我们发现糖尿病小鼠的卵母细胞在体外暴露于褪黑激素可以减轻异常的卵母细胞成熟能力。值得注意的是，褪黑激素的补充减弱缺陷，纺锤体组织和染色体排列介导的表达TPX2和pericentrin的本地化。重要的是，褪黑激素消除了活性氧的积累，并通过维持线粒体功能增加了糖尿病卵母细胞胞浆Ca 2+水平。此外，在糖尿病卵母细胞中，褪黑素暴露后通过降低LC 3 β表达逆转了自噬和凋亡的发生。总的来说，我们的研究结果提供了证据表明，褪黑激素补充剂可以保护卵母细胞免受母体糖尿病相关的减数分裂缺陷和卵子质量差的影响，为辅助生殖技术中改善卵母细胞质量提供了一种潜在的策略。
Maternal diabetes has been widely reported to adversely affect oocyte quality. Although various molecules and pathways may be involved in this process, strategies to prevent maternal diabetes-induced deterioration of oocyte quality remain unexplored. Melatonin is synthesized by the pineal gland and has been shown to have beneficial effects on oocyte quality owing to its antioxidative function. In the present study, we found that the exposure of oocytes of diabetic mice to melatonin,in vitro, alleviated aberrant oocyte maturation competence. Notably, melatonin supplementation attenuated defects in spindle organization and chromosome alignment by mediating the expression of TPX2 and pericentrin localization. Importantly, melatonin eliminated the accumulation of reactive oxygen species and increased the cytosolic Ca2+levels in diabetic oocytes by maintaining mitochondrial function. Moreover, the occurrence of autophagy and apoptosis was reversed in diabetic oocytes after melatonin exposure via decreased LC3β expression. Collectively, our findings provide evidence that melatonin supplementation can protect oocytes from maternal diabetes-related meiotic defects and poor egg quality, providing a potential strategy for improving oocyte quality in assisted reproductive technologies.