VqWRKY56 interacts with VqbZIPC22 in grapevine to promote proanthocyanidin biosynthesis and increase resistance to powdery mildew
VqWRKY56 interacts with VqbZIPC22 in grapevine to promote proanthocyanidin biosynthesis and increase resistance to powdery mildew
复制标题
VqWRKY56与葡萄中的VqbZIPC22相互作用促进原花青素生物合成并增强对白粉病的抗性
DOI：
10.1111/nph.18688
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发表时间：
2023
期刊：
影响因子：
9.4
通讯作者：
Xiping Wang
中科院分区：
文献类型：
--
作者：
Ya Wang;Xianhang Wang;Jinghao Fang;Xiaoxiao Yan;Mingxing Tu;Hui Liu;Zhengda Zhang;Zhi Li;Min Gao;Hua Lu;Yuejin Wang;Xiping Wang
白粉病是世界性栽培葡萄上的一种严重的真菌病害。原花青素(Pas)在葡萄对真菌病原菌的抗性中起着重要的作用，但对PA介导的葡萄对PM的抗性知之甚少。我们从葡萄中鉴定出一个WRKY转录因子VqWRKY56，该转录因子的表达受到PM的显著诱导。VqWRKY56在葡萄中的过表达增加了PA的含量，降低了对PM的敏感性此外，转基因植株表现出更多的细胞死亡，水杨酸和活性氧的积累增加。VqWRKY56inV的瞬时静音。五角形和五角形。维尼菲减少PA蓄积，增加PM的易感性VqWRKY56与VqbZIPC22在体外和体内均有相互作用。VqWRKY56蛋白可与VvCHS3、VvLAR1和VvANR启动子结合，VqbZIPC22可与VvANR启动子结合。VqWRKY56和VqbZIPC22共表达显著提高了VvCHS3、VvLAR1和VvANR基因的转录水平。最后给出了VqbZIPC22inV的暂态OE。Vinifera促进了PA的积累，提高了对PM的抗性，同时瞬时沉默Inv。本研究为进一步研究VqWRKY56对葡萄PA的调控机制提供了新的思路，并为进一步进行PA生物合成代谢工程以提高对PM的抗性奠定了基础。
Powdery mildew (PM) is a severe fungal disease of cultivated grapevine world‐wide. Proanthocyanidins (PAs) play an important role in resistance to fungal pathogens; however, little is known about PA‐mediated PM resistance in grapevine.We identified a WRKY transcription factor,VqWRKY56, fromVitis quinquangularis, the expression of which was significantly induced by PM.Overexpression (OE) ofVqWRKY56inVitis viniferaincreased PA content and reduced susceptibility to PM. Furthermore, the transgenic plants showed more cell death and increased accumulation of salicylic acid and reactive oxygen species. Transient silencing ofVqWRKY56inV. quinquangularisandV. viniferareduced PA accumulation and increased the susceptibility to PM. VqWRKY56 interacted with VqbZIPC22 in vitro and in planta. The protein VqWRKY56 can bind toVvCHS3,VvLAR1, andVvANRpromoters, and VqbZIPC22 can bind toVvANRpromoter. Co‐expression ofVqWRKY56andVqbZIPC22significantly increased the transcript level ofVvCHS3,VvLAR1, andVvANRgenes. Finally, transient OE ofVqbZIPC22inV. viniferapromoted PA accumulation and improved resistance to PM, while transient silencing inV. quinquangularishad the opposite effect.Our study provides new insights into the mechanism of PA regulation byVqWRKY56in grapevine and provides a basis for further metabolic engineering of PA biosynthesis to improve PM resistance.