Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice
Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice
复制标题
DOI：
10.1016/j.plantsci.2004.08.001
复制
发表时间：
2005-01-01
期刊：
影响因子：
5.2
通讯作者：
Graner, A
中科院分区：
文献类型：
--
作者：
Varshney, RK;Sigmund, R;Graner, A
最近增加的可用性表达序列标签（EST）的数据，促进了微卫星或简单重复序列（SSR）标记在一些植物物种组，包括谷物的发展。由于这些SSR来自EST/基因（EST-SSR），它们表现出更高的潜力，通过交叉扩增在相关物种中的转移比SSR标记产生的基因组DNA文库。在这项研究中，一个子集的165个EST-SSR标记，从共185分配到大麦的遗传图谱进行了检查，转移到小麦，黑麦和水稻。比例较高，即，78.2%的大麦标记在小麦中扩增，其次是75.2%的黑麦和42.4%的水稻。将185个定位的大麦EST-SSR位点与1,369,182个公开的谷类ESTs进行电子比对，发现与小麦（93.5%）、黑麦（37.3%）、水稻（57.3%）的ESTs具有显著的同源性。高粱（51.9%）和玉米（51.9%）。大麦ESTs与两种模式双子叶植物拟南芥和苜蓿的379，944个ESTs的序列相似性表明大麦标记理论上可转移到双子叶植物中，尽管频率较低（拟南芥中为9.7%，苜蓿中为8.6%）。在计算机比较映射（序列比较）映射大麦SSR-EST对黑麦，小麦和水稻的映射数据表明大麦SSR-EST在各自的物种中的直系同源物的存在。此外，9大麦EST-SSR实验定位到黑麦遗传连锁图谱，都可以定位在预期的正交区域相比，他们在大麦中的位置。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Recent increase in the availability of expressed sequence tag (EST) data has facilitated the development of microsatellite or simple sequence repeat (SSR) markers in a number of plant species groups, including cereals. As these SSRs are derived from ESTs/genes (EST-SSRs), they exhibit a higher potential for transfer through cross-amplification in related species than SSR markers generated from genomic DNA libraries. In this study, a sub-set of 165 EST-SSR markers from a total of 185 assigned to the genetic map of barley was examined for transferability to wheat, rye and rice. A higher proportion, i.e., 78.2% of barley markers showed amplification in wheat followed by 75.2% in rye and 42.4% in rice. Furthermore, in silico comparison of SSR-ESTs (ESTs containing SSRs) corresponding to 185 mapped barley EST-SSR loci against 1,369,182 publicly available cereal ESTs showed significant homology with ESTs of wheat (93.5%), rye (37.3%), rice (57.3%). sorghum (51.9%) and maize (51.9%). Sequence similarity of the barley ESTs with 379,944 ESTs of the two model dicot species, Arabidopsis and Medicago suggested theoretical transferability of barley markers into dicot species although at low frequency (9.7% in Arabidopsis and 8.6% in Medicago). In silico comparative mapping (sequence comparison) of mapped barley SSR-ESTs against the mapping data of rye, wheat and rice indicated the presence of orthologues of the barley SSR-ESTs in the respective species. Furthermore, nine barley EST-SSRs were experimentally mapped to a rye genetic linkage map and all could be located in the expected orthologous region compared to their position in barley. (C) 2004 Elsevier Ireland Ltd. All rights reserved.