Proteomic Analysis of Differentially Expressed Proteins Involved in Peel Senescence in Harvested Mandarin Fruit
Proteomic Analysis of Differentially Expressed Proteins Involved in Peel Senescence in Harvested Mandarin Fruit
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柑橘果实果皮衰老相关差异表达蛋白的蛋白质组学分析
DOI：
10.3389/fpls.2016.00725
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发表时间：
2016-05-31
影响因子：
5.6
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
--
作者：
Li, Taotao;Zhang, Jingying;Jiang, Yueming
柑橘（Citrus reticulata）是一种非跃变型水果，是世界上重要的经济水果。非跃变型果实衰老的机理还不清楚。本研究采用凝胶电泳技术结合LC-ESI-MS/MS技术，对贮藏18 d的沙糖桔果皮衰老过程中的蛋白质组变化进行了研究。在贮藏过程中，果实逐渐衰老，呼吸速率下降，叶绿素降解增加，膜完整性破坏。鉴定了63个丰度差异显著的蛋白质点。上调表达的蛋白主要与细胞壁降解、脂质降解、蛋白质降解、衰老相关转录因子和转录相关蛋白有关。相反，大多数与ATP合成和活性氧清除相关的蛋白质在果皮衰老过程中显着下调。在果皮衰老过程中，三种硫氧还蛋白和三种Ca 2+信号相关蛋白表达显著上调。柑橘果皮衰老与能量供应效率降低、抗氧化能力下降、蛋白质和脂质降解增加有关。此外，Ca ~（2+）信号和转录因子的激活可能参与细胞壁降解和初级或次级代谢。
Mandarin (Citrus reticulata), a non-climacteric fruit, is an economically important fruit worldwide. The mechanism underlying senescence of non-climacteric fruit is poorly understood. In this study, a gel-based proteomic study followed by LC-ESI-MS/MS analysis was carried out to investigate the proteomic changes involved in peel senescence in harvested mandarin “Shatangju” fruit stored for 18 days. Over the course of the storage period, the fruit gradually senesced, accompanied by a decreased respiration rate and increased chlorophyll degradation and disruption of membrane integrity. Sixty-three proteins spots that showed significant differences in abundance were identified. The up-regulated proteins were mainly associated with cell wall degradation, lipid degradation, protein degradation, senescence-related transcription factors, and transcription-related proteins. In contrast, most proteins associated with ATP synthesis and scavenging of reactive oxygen species were significantly down-regulated during peel senescence. Three thioredoxin proteins and three Ca2+ signaling-related proteins were significantly up-regulated during peel senescence. It is suggested that mandarin peel senescence is associated with energy supply efficiency, decreased antioxidant capability, and increased protein and lipid degradation. In addition, activation of Ca2+ signaling and transcription factors might be involved in cell wall degradation and primary or secondary metabolism.