Open-source, community-driven microfluidics with Metafluidics
Open-source, community-driven microfluidics with Metafluidics
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DOI：
10.1038/nbt.3873
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发表时间：
2017-06-01
影响因子：
46.9
通讯作者：
Carr, Peter A.
中科院分区：
文献类型：
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作者：
Kong, David S.;Thorsen, Todd A.;Carr, Peter A.
微流控设备有可能实现生物实验的自动化和微型化，但设备设计的开源共享落后于软件等其他资源的共享。合成生物学家已经将微流控技术用于DNA组装、无细胞表达和细胞培养，但费用、设备复杂性和对定制设置的依赖阻碍了它们的广泛采用。我们介绍了Metafluidics，这是一个开源的、社区驱动的存储库，它托管了数字设计文件、组装规范和开源软件，使用户能够构建、配置和操作微流体设备。我们使用Metafluidics分享微流体环形混合器装置和32通道桌面微流体控制器的设计和制造说明。这种设备和控制器被应用于使用标准的DNA组装方法来构建基因电路，包括连接、Gateway、Gibson和Golden Gate。Metafluidics旨在使工程师、DIY爱好者和其他制造技能有限的非传统参与者能够为微流体研究做出贡献。
Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present Metafluidics, an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build, configure, and operate a microfluidic device. We use Metafluidics to share designs and fabrication instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic controller. This device and controller are applied to build genetic circuits using standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate. Metafluidics is intended to enable a broad community of engineers, DIY enthusiasts, and other nontraditional participants with limited fabrication skills to contribute to microfluidic research.